BMRB Entry 52372
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52372
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Brigandat, Louis; Laux, Maelys; Marteau, Caroline; Cole, Laura; Bockmann, Anja; Lecoq, Lauriane; Forgeron, Marie-Laure; Callon, Morgane. "NMR side-chain assignments of the Crimean\u2013Congo hemorrhagic fever virus glycoprotein n cytosolic domain" Magnetic Resonance 5, 95-101 (2024).
Assembly members:
entity_1, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: Orthonairovirus haemorrhagiae Taxonomy ID: 3052518 Superkingdom: Viruses Kingdom: not available Genus/species: Orthonairovirus haemorrhagiae
Experimental source: Production method: cell free synthesis Host organism: wheat germ - cell free Vector: pEU-E01-MCS
Entity Sequences (FASTA):
entity_1: MKPQTCTICETTPVNAIDAE
MHDLNCSYNICPYCASRLTS
DGLARHVIQCPKRKEKVEET
ELYLNLERIGGWSHPQFEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 244 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 491 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail | 1 |
Entities:
Entity 1, Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail 79 residues - Formula weight is not available
| 1 | MET | LYS | PRO | GLN | THR | CYS | THR | ILE | CYS | GLU | ||||
| 2 | THR | THR | PRO | VAL | ASN | ALA | ILE | ASP | ALA | GLU | ||||
| 3 | MET | HIS | ASP | LEU | ASN | CYS | SER | TYR | ASN | ILE | ||||
| 4 | CYS | PRO | TYR | CYS | ALA | SER | ARG | LEU | THR | SER | ||||
| 5 | ASP | GLY | LEU | ALA | ARG | HIS | VAL | ILE | GLN | CYS | ||||
| 6 | PRO | LYS | ARG | LYS | GLU | LYS | VAL | GLU | GLU | THR | ||||
| 7 | GLU | LEU | TYR | LEU | ASN | LEU | GLU | ARG | ILE | GLY | ||||
| 8 | GLY | TRP | SER | HIS | PRO | GLN | PHE | GLU | LYS |
Samples:
sample_1: Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail, [U-100% 13C; U-100% 15N], 150 uM; phosphate buffer 50 mM; NaCl 50 mM; desthiobiotin 2.5 mM; zinc sulfate 100 uM
sample_2: Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail, [U-13C; U-15N; U-2H], 109 uM; phosphate buffer 50 mM; NaCl 50 mM; desthiobiotin 2.5 mM; zinc sulfate 100 uM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR vversion 3 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 950 MHz
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks