BMRB Entry 52368

Title:
The backbone assignment of FtsZ C-terminal disordered tail
Deposition date:
2024-03-26
Original release date:
2024-07-22
Authors:
Qu, Xiaozhan
Citation:

Citation: Qu, Xiaozhan; Zhao, Shuo; Wan, Chanjuan; Zhu, Lei; Ji, Tuo; Rossi, Paolo; Wang, Junfeng; Kalodimos, Charalampos; Wang, Chao; Xu, Weiya; Huang, Chengdong. "Structural basis for the dynamic chaperoning of disordered clients by Hsp90"  Nat. Struct. Mol. Biol. ., .-. (2024).
PubMed: 38890550

Assembly members:

Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts117
15N chemical shifts58
1H chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FtsZ C-terminal disordered tail1

Entities:

Entity 1, FtsZ C-terminal disordered tail 71 residues - Formula weight is not available

1   THRGLYILEGLYMETASPLYSARGPROGLU
2   ILETHRLEUVALTHRASNLYSGLNVALGLN
3   GLNPROVALMETASPARGTYRGLNGLNHIS
4   GLYMETALAPROLEUTHRGLNGLUGLNLYS
5   PROVALALALYSVALVALASNASPASNALA
6   PROGLNTHRALALYSGLUPROASPTYRLEU
7   ASPILEPROALAPHELEUARGLYSGLNALA
8   ASP

Samples:

sample_1: FtsZ C-terminal disordered tail, [U-99% 13C; U-99% 15N], 500 uM; Phosphate 50 mM; NaCl 100 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY - data analysis

NMRViewJ - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks