BMRB Entry 52368

Name: The backbone assignment of FtsZ C-terminal disordered tail
Published: 2024-07-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52368

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The backbone assignment of FtsZ C-terminal disordered tail

Deposition date:

2024-03-26

Original release date:

2024-07-22

Authors:

Qu, Xiaozhan

Citation:

Citation: Qu, Xiaozhan; Zhao, Shuo; Wan, Chanjuan; Zhu, Lei; Ji, Tuo; Rossi, Paolo; Wang, Junfeng; Kalodimos, Charalampos; Wang, Chao; Xu, Weiya; Huang, Chengdong. "Structural basis for the dynamic chaperoning of disordered clients by Hsp90" Nat. Struct. Mol. Biol. ., .-. (2024).
PubMed: 38890550

Assembly members:

Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TGIGMDKRPEITLVTNKQVQ QPVMDRYQQHGMAPLTQEQK PVAKVVNDNAPQTAKEPDYL DIPAFLRKQAD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	117
15N chemical shifts	58
1H chemical shifts	58

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FtsZ C-terminal disordered tail	1

Entities:

Entity 1, FtsZ C-terminal disordered tail 71 residues - Formula weight is not available

1

THR

GLY

ILE

GLY

MET

ASP

LYS

ARG

PRO

GLU

2

ILE

THR

LEU

VAL

THR

ASN

LYS

GLN

VAL

GLN

3

GLN

PRO

VAL

MET

ASP

ARG

TYR

GLN

HIS

4

GLY

MET

ALA

PRO

LEU

THR

GLN

GLU

GLN

LYS

5

PRO

VAL

ALA

LYS

VAL

ASN

ASP

ASN

ALA

6

PRO

GLN

THR

ALA

LYS

GLU

PRO

ASP

TYR

LEU

7

ASP

ILE

PRO

ALA

PHE

LEU

ARG

LYS

GLN

ALA

8

ASP

Samples:

sample_1: FtsZ C-terminal disordered tail, [U-99% 13C; U-99% 15N], 500 uM; Phosphate 50 mM; NaCl 100 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - data analysis

NMRViewJ - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks