BMRB Entry 52355
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52355
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Citation: Wieruszewska, Julia; Pawlowicz, Aleksandra; Polomska, Ewa; Pasternak, Karol; Gdaniec, Zofia; Andralojc, Witold. "The 8-17 DNAzyme can operate in a single active structure regardless of metal ion cofactor" Nat. Commun. 15, 4218-4218 (2024).
PubMed: 38760331
Assembly members:
entity_1, polymer, 35 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: CGCCGGGGTCGAAGACTGCC
AGCGGCTCGACGGCG
| Data type | Count |
| 1H chemical shifts | 2071 |
| 31P chemical shifts | 180 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 8-17 DNAzyme | 1 |
Entities:
Entity 1, 8-17 DNAzyme 35 residues - Formula weight is not available
| 1 | DC | DG | DC | DC | DG | DG | DG | DG | DT | DC | ||||
| 2 | DG | DA | DA | DG | DA | DC | DT | DG | DC | DC | ||||
| 3 | DA | DG | DC | DG | DG | DC | DT | DC | DG | DA | ||||
| 4 | DC | DG | DG | DC | DG |
Samples:
sample_1: 8-17 DNAzyme 1.5 mM; sodium cacodylate 10 mM; NaCl 400 mM
sample_2: 8-17 DNAzyme 1.5 mM; sodium cacodylate 10 mM; ZnCl2 1.5 mM
sample_3: 8-17 DNAzyme 1.5 mM; sodium cacodylate 10 mM; MgCl2 7.5 mM
sample_4: 8-17 DNAzyme 1.5 mM; sodium cacodylate 10 mM; MgCl2 3.5 mM
sample_5: 8-17 DNAzyme 1.5 mM; sodium cacodylate 10 mM; NaCl 400 mM; ZnCl2 1.5 mM
sample_6: 8-17 DNAzyme 1.5 mM; sodium cacodylate 10 mM; NaCl 400 mM; ZnCl2 3.0 mM
sample_7: 8-17 DNAzyme 1.5 mM; sodium cacodylate 10 mM; NaCl 400 mM; ZnCl2 4.5 mM
sample_8: 8-17 DNAzyme 1.5 mM; sodium cacodylate 10 mM; ZnCl2 3.0 mM
sample_9: 8-17 DNAzyme 1.5 mM; sodium cacodylate 10 mM; ZnCl2 4.5 mM
sample_conditions_1: ionic strength: 0.4 M; pH: 6.0; pressure: 1 atm; temperature: 293 K
sample_conditions_2: ionic strength: 0.01 M; pH: 6.0; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_3 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_4 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_5 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_6 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_7 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_8 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_9 | isotropic | sample_conditions_2 |
Software:
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 700 MHz