BMRB Entry 52351

Name: Backbone 1H, 13C and 15N chemical shift assignments of E. coli C6-crypto-AcpP.
Published: 2024-06-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52351

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C and 15N chemical shift assignments of E. coli C6-crypto-AcpP.

Deposition date:

2024-03-13

Original release date:

2024-06-19

Authors:

Kalaj, Brianna; Burkart, Michael; Deshmukh, Lalit

Citation:

Citation: Kalaj, Brianna; La Clair, James; Shen, Yang; Schwieters, Charles; Deshmukh, Lalit; Burkart, Michael. "Quantitative Characterization of Chain-Flipping of Acyl Carrier Protein of Escherichia coli Using Chemical Exchange NMR" J. Am. Chem. Soc. 146, 18650-18660 (2024).
PubMed: 38875499

Assembly members:

Assembly members:
entity_1, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSTIEERVKKIIGEQLGVKQ EEVTNNASFVEDLGADSLDT VELVMALEEEFDTEIPDEEA EKITTVQAAIDYINGHQALE HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	223
15N chemical shifts	72
1H chemical shifts	72

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C6-crypto-AcpP	1

Entities:

Entity 1, C6-crypto-AcpP 86 residues - Formula weight is not available

1

MET

SER

THR

ILE

GLU

ARG

VAL

LYS

2

ILE

GLY

GLU

GLN

LEU

GLY

VAL

LYS

GLN

3

GLU

VAL

THR

ASN

ALA

SER

PHE

VAL

4

GLU

ASP

LEU

GLY

ALA

ASP

SER

LEU

ASP

THR

5

VAL

GLU

LEU

VAL

MET

ALA

LEU

GLU

6

PHE

ASP

THR

GLU

ILE

PRO

ASP

GLU

ALA

7

GLU

LYS

ILE

THR

VAL

GLN

ALA

ILE

8

ASP

TYR

ILE

ASN

GLY

HIS

GLN

ALA

LEU

GLU

9

HIS

Samples:

sample_1: C6-crypto-AcpP, [U-100% 13C; U-100% 15N], 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - data analysis

CcpNMR - data analysis

NMR spectrometers:

Bruker Avance 600 MHz