BMRB Entry 52349

Name: Isoform-specific C-terminal phosphorylation drives autoinhibition of Casein Kinase 1
Published: 2024-08-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52349

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Isoform-specific C-terminal phosphorylation drives autoinhibition of Casein Kinase 1

Deposition date:

2024-03-12

Original release date:

2024-08-28

Authors:

Harold, Rachel; Lee, Hsiau-Wei; Partch, Carrie

Citation:

Citation: Harold, Rachel; Tulsian, Nikhil; Narasimamurthy, Rajesh; Yaitanes, Noelle; Ayala Hernandez, Maria; Lee, Hsiau-Wei; Crosby, Priya; Virshup, David; Partch, Carrie. "Isoform-specific C-terminal phosphorylation drives autoinhibition of Casein Kinase 1" bioRxiv ., .-. (2024).
PubMed: 39131317

Assembly members:

Assembly members:
entity_1, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMDPEFWFGASRAADDAER ERRDREERLRHSRNPATRGL PSTASGRLRGTQEVAPPTPL TPTSHTANTSPRPVSGMERE RKVSMRLHRGAPVNISSSDL TGRQDTSRMSTSQIPGRVAS SGLQSVVHR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	397
15N chemical shifts	120
1H chemical shifts	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CK1d1 tail	1

Entities:

Entity 1, CK1d1 tail 129 residues - Formula weight is not available

1

GLY

ALA

MET

ASP

PRO

GLU

PHE

TRP

PHE

GLY

2

ALA

SER

ARG

ALA

ASP

ALA

GLU

ARG

3

GLU

ARG

ASP

ARG

GLU

ARG

LEU

ARG

4

HIS

SER

ARG

ASN

PRO

ALA

THR

ARG

GLY

LEU

5

PRO

SER

THR

ALA

SER

GLY

ARG

LEU

ARG

GLY

6

THR

GLN

GLU

VAL

ALA

PRO

THR

PRO

LEU

7

THR

PRO

THR

SER

HIS

THR

ALA

ASN

THR

SER

8

PRO

ARG

PRO

VAL

SER

GLY

MET

GLU

ARG

GLU

9

ARG

LYS

VAL

SER

MET

ARG

LEU

HIS

ARG

GLY

10

ALA

PRO

VAL

ASN

ILE

SER

ASP

LEU

11

THR

GLY

ARG

GLN

ASP

THR

SER

ARG

MET

SER

12

THR

SER

GLN

ILE

PRO

GLY

ARG

VAL

ALA

SER

13

SER

GLY

LEU

GLN

SER

VAL

HIS

ARG

Samples:

sample_1: CK1d1 tail, [U-100% 13C; U-100% 15N], 500 uM; D2O 10%; EDTA 1 mM; sodium chloride 100 mM; beta-mercaptoethanol 5 mM; mops 25 mM; tween 0.05%; Magnesium chloride 11 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 13C-15N CON	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HACANCACON	sample_1	isotropic	sample_conditions_1
3D HACANCANCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMRPipe - processing

POKY - chemical shift assignment

NMRDraw - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks