BMRB Entry 52346

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52346

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Title:
CPEB4 N-terminal domain isoform lacking microexon 4
Deposition date:
2024-03-07
Original release date:
2024-09-05
Authors:
Garcia-Cabau, Carla; Garcia, Jesus; Salvatella, Xavier
Citation:

Citation: Garcia-Cabau, Carla; Bartomeu, Anna; Tesei, Giulio; Cheung, Kai; Pose-Utrilla, Julia; Pico, Sara; Balaceanu, Andreea; Duran-Arque, Berta; Fernandez-Alfara, Marcos; Martin, Judit; De Pace, Cesare; Ruiz-Perez, Lorena; Garcia, Jesus; Battaglia, Giuseppe; Lucas, Jose; Hervas, Ruben; Lindorff-Larsen, Kresten; Mendez, Raul; Salvatella, Xavier. "kinetic stabilization of translation-repression condensates by a neuron-specific microexon"  Nature ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 440 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGDYGFGVLVQSNTGNKSAF PVRFHPHLQPPHHHQNATPS PAAFINNNTAANGSSAGSAW LFPAPATHNIQDEILGSEKA KSQQQEQQDPLEKQQLSPSP GQEAGILPETEKAKSEENQG DNSSENGNGKEKIRIESPVL TGFDYQEATGLGTSTQPLTS SASSLTGFSNWSAAIAPSSS TIINEDASFFHQGGVPAASA NNGALLFQNFPHHVSPGFGG SFSPQIGPLSQHHPHHPHFQ HHHSQHQQQRRSPASPHPPP FTHRNAAFNQLPHLANNLNK PPSPWSSYQSPSPTPSSSWS PGGGGYGGWGGSQGRDHRRG LNGGITPLNSISPLKKNFAS NHIQLQKYARPSSAFAPKSW MEDSLNRADNIFPFPDRPRT FDMHSLESSLIDIMRAENDT IKGQSSLFPMEDGFLDDGRG DQPLHSGLGSPHCFSHQNGE

Data sets:
Data typeCount
13C chemical shifts1194
15N chemical shifts374
1H chemical shifts374

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human CPEB4-NTD lacking microexon 41

Entities:

Entity 1, human CPEB4-NTD lacking microexon 4 440 residues - Formula weight is not available

1   METGLYASPTYRGLYPHEGLYVALLEUVAL
2   GLNSERASNTHRGLYASNLYSSERALAPHE
3   PROVALARGPHEHISPROHISLEUGLNPRO
4   PROHISHISHISGLNASNALATHRPROSER
5   PROALAALAPHEILEASNASNASNTHRALA
6   ALAASNGLYSERSERALAGLYSERALATRP
7   LEUPHEPROALAPROALATHRHISASNILE
8   GLNASPGLUILELEUGLYSERGLULYSALA
9   LYSSERGLNGLNGLNGLUGLNGLNASPPRO
10   LEUGLULYSGLNGLNLEUSERPROSERPRO
11   GLYGLNGLUALAGLYILELEUPROGLUTHR
12   GLULYSALALYSSERGLUGLUASNGLNGLY
13   ASPASNSERSERGLUASNGLYASNGLYLYS
14   GLULYSILEARGILEGLUSERPROVALLEU
15   THRGLYPHEASPTYRGLNGLUALATHRGLY
16   LEUGLYTHRSERTHRGLNPROLEUTHRSER
17   SERALASERSERLEUTHRGLYPHESERASN
18   TRPSERALAALAILEALAPROSERSERSER
19   THRILEILEASNGLUASPALASERPHEPHE
20   HISGLNGLYGLYVALPROALAALASERALA
21   ASNASNGLYALALEULEUPHEGLNASNPHE
22   PROHISHISVALSERPROGLYPHEGLYGLY
23   SERPHESERPROGLNILEGLYPROLEUSER
24   GLNHISHISPROHISHISPROHISPHEGLN
25   HISHISHISSERGLNHISGLNGLNGLNARG
26   ARGSERPROALASERPROHISPROPROPRO
27   PHETHRHISARGASNALAALAPHEASNGLN
28   LEUPROHISLEUALAASNASNLEUASNLYS
29   PROPROSERPROTRPSERSERTYRGLNSER
30   PROSERPROTHRPROSERSERSERTRPSER
31   PROGLYGLYGLYGLYTYRGLYGLYTRPGLY
32   GLYSERGLNGLYARGASPHISARGARGGLY
33   LEUASNGLYGLYILETHRPROLEUASNSER
34   ILESERPROLEULYSLYSASNPHEALASER
35   ASNHISILEGLNLEUGLNLYSTYRALAARG
36   PROSERSERALAPHEALAPROLYSSERTRP
37   METGLUASPSERLEUASNARGALAASPASN
38   ILEPHEPROPHEPROASPARGPROARGTHR
39   PHEASPMETHISSERLEUGLUSERSERLEU
40   ILEASPILEMETARGALAGLUASNASPTHR
41   ILELYSGLYGLNSERSERLEUPHEPROMET
42   GLUASPGLYPHELEUASPASPGLYARGGLY
43   ASPGLNPROLEUHISSERGLYLEUGLYSER
44   PROHISCYSPHESERHISGLNASNGLYGLU

Samples:

sample_1: human CPEB4-NTD lacking microexon 4, [U-100% 13C; U-100% 15N], 200 uM; D2O, [U-100% 2H], 7%; sodium phosphate 20 mM; sodium azide 0.05%; TCEP 1 mM; DSS 2.5 uM; urea 4 M

sample_conditions_1: ionic strength: 20 mM; pH: 7; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N BEST-TROSYsample_1isotropicsample_conditions_1
3D BEST-TROSY HNCOsample_1isotropicsample_conditions_1
3D BEST-TROSY HN(CA)COsample_1isotropicsample_conditions_1
3D BEST-TROSY HNCAsample_1isotropicsample_conditions_1
3D BEST-TROSY HNCACBsample_1isotropicsample_conditions_1
3D BEST-TROSY HN(CO)CACBsample_1isotropicsample_conditions_1
3D (H)N(CA)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.8 - collection

qMDD v3.2 - processing

NMRPipe v9.5 - processing

CcpNMR v2.3.0 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks