BMRB Entry 52344

Title:
Ubiqitin L50A mutant pressure unfolded
Deposition date:
2024-03-03
Original release date:
2024-07-22
Authors:
Masoumzadeh, Elahe; Ying, Jinfa; Baber, James; Anfinrud, Philip; Bax, Ad
Citation:

Citation: Masoumzadeh, Elahe; Ying, Jinfa; Baber, James; Anfinrud, Philip; Bax, Ad. "Proline Peptide Bond Isomerization in Ubiquitin Under Folding and Denaturing Conditions by Pressure-Jump NMR"  J. Mol. Biol. 436, 168587-168587 (2024).
PubMed: 38663546

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts72
15N chemical shifts72
1H chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubq L50A denatured1

Entities:

Entity 1, Ubq L50A denatured 76 residues - Formula weight is not available

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNALA
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: Ubq L50A denatured monomer, [U-2H; U-15N], 400 uM; sodium phosphate 20 mM; EDTA 1 mM; benzamidine 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.4; pressure: 2500 bar; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

ccpn analysis - chemical shift assignment

NMRPipe - data analysis

SMILE - data analysis

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks