BMRB Entry 52340

Name: 4E-tau(297-407) in vitro AD C-shaped
Published: 2024-07-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52340

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

4E-tau(297-407) in vitro AD C-shaped

Deposition date:

2024-02-26

Original release date:

2024-07-22

Authors:

Duan, Pu; El Mammeri, Nadia; Hong, Mei

Citation:

Citation: Duan, Pu; El Mammeri, Nadia; Hong, Mei. "Milligram-scale assembly and NMR fingerprint of tau fibrils adopting the Alzheimer's disease fold" J. Biol. Chem. 300, 107326-107326 (2024).
PubMed: 38679331

Assembly members:

Assembly members:
entity_1, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MIKHVPGGGSVQIVYKPVDL SKVTSKCGSLGNIHHKPGGG QVEVKSEKLDFKDRVQSKIG SLDNITHVPGGGNKKIETHK LTFRENAKAKTDHGAEIVYK EPVVEGDEEPRH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	225
15N chemical shifts	55

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	4E-tau(297-407)	1

Entities:

Entity 1, 4E-tau(297-407) 112 residues - Formula weight is not available

1

MET

ILE

LYS

HIS

VAL

PRO

GLY

SER

2

VAL

GLN

ILE

VAL

TYR

LYS

PRO

VAL

ASP

LEU

3

SER

LYS

VAL

THR

SER

LYS

CYS

GLY

SER

LEU

4

GLY

ASN

ILE

HIS

LYS

PRO

GLY

5

GLN

VAL

GLU

VAL

LYS

SER

GLU

LYS

LEU

ASP

6

PHE

LYS

ASP

ARG

VAL

GLN

SER

LYS

ILE

GLY

7

SER

LEU

ASP

ASN

ILE

THR

HIS

VAL

PRO

GLY

8

GLY

ASN

LYS

ILE

GLU

THR

HIS

LYS

9

LEU

THR

PHE

ARG

GLU

ASN

ALA

LYS

ALA

LYS

10

THR

ASP

HIS

GLY

ALA

GLU

ILE

VAL

TYR

LYS

11

GLU

PRO

VAL

GLU

GLY

ASP

GLU

PRO

12

ARG

HIS

Samples:

sample_1: 4E-Tau (297-407), [U-100% 13C; U-100% 15N], 50 % w/w; potassium phosphate buffer 10 mM; magnesium chloride 100 mM

sample_conditions_1: ionic strength: 0.31 M; pH: 7.5; pressure: 1 atm; temperature: 280 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-detected NCACB	sample_1	isotropic	sample_conditions_1
3D NCACX	sample_1	isotropic	sample_conditions_1
3D NCOCX	sample_1	isotropic	sample_conditions_1
3D CONCA	sample_1	isotropic	sample_conditions_1
2D 13C-detected CORD	sample_1	isotropic	sample_conditions_1
2D 13C-detected NCA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.3 - processing

CcpNmr Analysis v2.4.2 - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 800 MHz