BMRB Entry 52340

Title:
4E-tau(297-407) in vitro AD C-shaped
Deposition date:
2024-02-26
Original release date:
2024-07-22
Authors:
Duan, Pu; El Mammeri, Nadia; Hong, Mei
Citation:

Citation: Duan, Pu; El Mammeri, Nadia; Hong, Mei. "Milligram-scale assembly and NMR fingerprint of tau fibrils adopting the Alzheimer's disease fold"  J. Biol. Chem. 300, 107326-107326 (2024).
PubMed: 38679331

Assembly members:

Assembly members:
entity_1, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET-28a

Data sets:
Data typeCount
13C chemical shifts225
15N chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
14E-tau(297-407)1

Entities:

Entity 1, 4E-tau(297-407) 112 residues - Formula weight is not available

1   METILELYSHISVALPROGLYGLYGLYSER
2   VALGLNILEVALTYRLYSPROVALASPLEU
3   SERLYSVALTHRSERLYSCYSGLYSERLEU
4   GLYASNILEHISHISLYSPROGLYGLYGLY
5   GLNVALGLUVALLYSSERGLULYSLEUASP
6   PHELYSASPARGVALGLNSERLYSILEGLY
7   SERLEUASPASNILETHRHISVALPROGLY
8   GLYGLYASNLYSLYSILEGLUTHRHISLYS
9   LEUTHRPHEARGGLUASNALALYSALALYS
10   THRASPHISGLYALAGLUILEVALTYRLYS
11   GLUPROVALVALGLUGLYASPGLUGLUPRO
12   ARGHIS

Samples:

sample_1: 4E-Tau (297-407), [U-100% 13C; U-100% 15N], 50 % w/w; potassium phosphate buffer 10 mM; magnesium chloride 100 mM

sample_conditions_1: ionic strength: 0.31 M; pH: 7.5; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-detected NCACBsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
3D CONCAsample_1isotropicsample_conditions_1
2D 13C-detected CORDsample_1isotropicsample_conditions_1
2D 13C-detected NCAsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.3 - processing

CcpNmr Analysis v2.4.2 - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz