BMRB Entry 5234
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5234
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Swarbrick, James; Bashtannyk, Tanya; Dixon, Mathew; Pau, Richard; Davidson, Barrie; Gooley, Paul. "Letter to the Editor: Backbone resonance assignment of the 2H, 13C, 15N
labelled 32kDa Central Domain of Escherichia coli TyrR" J. Biomol. NMR 22, 381-382 (2002).
PubMed: 12018490
Assembly members:
TyrR transcription factor central domain, polymer, 288 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
TyrR transcription factor central domain: MGRQLQNVAAQDVSAFSQIV
AVSPKMKHVVEQAQKLAMLS
APLLITGDTGTGKDLFAYAC
HQASPRAGKPYLALNCASIP
EDAVESELFGHAPEGKKGFF
EQANGGSVLLDEIGEMSPRM
QAKLLRFLNDGTFRRVGEDH
EVHVDVRVICATQKNLVELV
QKGMFREDLYYRLNVLTLNL
PPLRDCPQDIMPLTELFVAR
FADEQGVPLPKLAADLNTVL
TRYAWPGNVRQLKNAIYRAL
TQLDGYELRPQDILLPDYDA
ATVAVGEDAMEGSLDEITSR
LEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 672 |
| 15N chemical shifts | 246 |
| 1H chemical shifts | 247 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TyrR central domain | 1 |
Entities:
Entity 1, TyrR central domain 288 residues - Formula weight is not available
| 1 | MET | GLY | ARG | GLN | LEU | GLN | ASN | VAL | ALA | ALA | ||||
| 2 | GLN | ASP | VAL | SER | ALA | PHE | SER | GLN | ILE | VAL | ||||
| 3 | ALA | VAL | SER | PRO | LYS | MET | LYS | HIS | VAL | VAL | ||||
| 4 | GLU | GLN | ALA | GLN | LYS | LEU | ALA | MET | LEU | SER | ||||
| 5 | ALA | PRO | LEU | LEU | ILE | THR | GLY | ASP | THR | GLY | ||||
| 6 | THR | GLY | LYS | ASP | LEU | PHE | ALA | TYR | ALA | CYS | ||||
| 7 | HIS | GLN | ALA | SER | PRO | ARG | ALA | GLY | LYS | PRO | ||||
| 8 | TYR | LEU | ALA | LEU | ASN | CYS | ALA | SER | ILE | PRO | ||||
| 9 | GLU | ASP | ALA | VAL | GLU | SER | GLU | LEU | PHE | GLY | ||||
| 10 | HIS | ALA | PRO | GLU | GLY | LYS | LYS | GLY | PHE | PHE | ||||
| 11 | GLU | GLN | ALA | ASN | GLY | GLY | SER | VAL | LEU | LEU | ||||
| 12 | ASP | GLU | ILE | GLY | GLU | MET | SER | PRO | ARG | MET | ||||
| 13 | GLN | ALA | LYS | LEU | LEU | ARG | PHE | LEU | ASN | ASP | ||||
| 14 | GLY | THR | PHE | ARG | ARG | VAL | GLY | GLU | ASP | HIS | ||||
| 15 | GLU | VAL | HIS | VAL | ASP | VAL | ARG | VAL | ILE | CYS | ||||
| 16 | ALA | THR | GLN | LYS | ASN | LEU | VAL | GLU | LEU | VAL | ||||
| 17 | GLN | LYS | GLY | MET | PHE | ARG | GLU | ASP | LEU | TYR | ||||
| 18 | TYR | ARG | LEU | ASN | VAL | LEU | THR | LEU | ASN | LEU | ||||
| 19 | PRO | PRO | LEU | ARG | ASP | CYS | PRO | GLN | ASP | ILE | ||||
| 20 | MET | PRO | LEU | THR | GLU | LEU | PHE | VAL | ALA | ARG | ||||
| 21 | PHE | ALA | ASP | GLU | GLN | GLY | VAL | PRO | LEU | PRO | ||||
| 22 | LYS | LEU | ALA | ALA | ASP | LEU | ASN | THR | VAL | LEU | ||||
| 23 | THR | ARG | TYR | ALA | TRP | PRO | GLY | ASN | VAL | ARG | ||||
| 24 | GLN | LEU | LYS | ASN | ALA | ILE | TYR | ARG | ALA | LEU | ||||
| 25 | THR | GLN | LEU | ASP | GLY | TYR | GLU | LEU | ARG | PRO | ||||
| 26 | GLN | ASP | ILE | LEU | LEU | PRO | ASP | TYR | ASP | ALA | ||||
| 27 | ALA | THR | VAL | ALA | VAL | GLY | GLU | ASP | ALA | MET | ||||
| 28 | GLU | GLY | SER | LEU | ASP | GLU | ILE | THR | SER | ARG | ||||
| 29 | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: TyrR transcription factor central domain, [U-13C; U-2H; U-15N], 0.7 mM
Deuteration_1: pH: 7.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Trosy HNCA | not available | not available | not available |
| Trosy HNCACB | not available | not available | not available |
| Trosy HN(CO)CA | not available | not available | not available |
| Trosy HN(CO)CB | not available | not available | not available |
| Trosy HN(CA)CB | not available | not available | not available |
| 1H-15N NOESY_HSQC | not available | not available | not available |
Software:
NMRPipe - processing
XEASY v1.4 - assignments
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| DBJ | BAA14905 BAB35325 BAG76899 BAI25172 BAI30314 |
| EMBL | CAP75867 CAQ31828 CAQ98207 CAR02785 CAR07729 |
| GB | AAA24706 AAB25766 AAC74405 AAF28133 AAG56479 |
| REF | NP_287865 NP_309929 NP_415839 NP_707228 NP_753699 |
| SP | P07604 |
| AlphaFold | P07604 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks