BMRB Entry 52338

Title:
13C and 15N Chemical Shift Assignment for Abeta 40 amyloid fibrils polymorph 2 (P2)
Deposition date:
2024-02-21
Original release date:
2024-07-08
Authors:
Rodina, Natalia; Hornung, Simon; Sarkar, Riddhiman; Suladze, Saba; Peters, Carsten; Schmid, Philipp; Niu, Zheng; Haslbeck, Martin; Buchner, Johannes; Kapurniotu, Aphrodite; Reif, Bernd
Citation:

Citation: Rodina, Natalia; Hornung, Simon; Sarkar, Riddhiman; Suladze, Saba; Peters, Carsten; Schmid, Philipp; Niu, Zheng; Haslbeck, Martin; Buchner, Johannes; Kapurniotu, Aphrodite; Reif, Bernd. "Modulation of Alzheimer's Disease Ab40 Fibril Polymorphism by the Small Heat Shock Protein alpha-B-Crystallin"  J, Am, Chem, Soc, 146, 19077-19087 (2024).
PubMed: 38973199

Assembly members:

Assembly members:
entity_1, polymer, 40 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV

Data sets:
Data typeCount
13C chemical shifts104
15N chemical shifts25

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Abeta 40 amyloid fibrils1

Entities:

Entity 1, Abeta 40 amyloid fibrils 40 residues - Formula weight is not available

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAGLUASPVALGLYSERASNLYSGLYALA
4   ILEILEGLYLEUMETVALGLYGLYVALVAL

Samples:

sample_1: Abeta 40 peptide, [U-100% 13C; U-100% 15N], 50 uM; phosphate buffer 50 mM; NaCl 50 mM; NaN3 0.1%

sample_conditions_1: pH: 7.4; temperature: 283.15 K

Experiments:

NameSampleSample stateSample conditions
2D DARRsample_1isotropicsample_conditions_1
2D PDSDsample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 & 3.1.0 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 750 MHz