BMRB Entry 5233
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5233
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Espejo, F.; Green, M.; Preece, N.; Assa-Munt, N.. "Letter to the Editor: NMR assignment of human ASC2, a self contained protein interaction domain
involved in apoptosis and inflammation" J. Biomol. NMR 23, 151-152 (2002).
Assembly members:
ASC2, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ASC2: GPLGSPEFEAQGRAMGTKRE
AILKVLENLTPEELKKFKMK
LGTVPLREGFERIPRGALGQ
LDIVDLTDKLVASYYEDYAA
ELVVAVLRDMRMLEEAARLQ
RAA
| Data type | Count |
| 13C chemical shifts | 399 |
| 15N chemical shifts | 128 |
| 1H chemical shifts | 577 |
| coupling constants | 72 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ASC2 | 1 |
Entities:
Entity 1, ASC2 103 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | PRO | GLU | PHE | GLU | ALA | ||||
| 2 | GLN | GLY | ARG | ALA | MET | GLY | THR | LYS | ARG | GLU | ||||
| 3 | ALA | ILE | LEU | LYS | VAL | LEU | GLU | ASN | LEU | THR | ||||
| 4 | PRO | GLU | GLU | LEU | LYS | LYS | PHE | LYS | MET | LYS | ||||
| 5 | LEU | GLY | THR | VAL | PRO | LEU | ARG | GLU | GLY | PHE | ||||
| 6 | GLU | ARG | ILE | PRO | ARG | GLY | ALA | LEU | GLY | GLN | ||||
| 7 | LEU | ASP | ILE | VAL | ASP | LEU | THR | ASP | LYS | LEU | ||||
| 8 | VAL | ALA | SER | TYR | TYR | GLU | ASP | TYR | ALA | ALA | ||||
| 9 | GLU | LEU | VAL | VAL | ALA | VAL | LEU | ARG | ASP | MET | ||||
| 10 | ARG | MET | LEU | GLU | GLU | ALA | ALA | ARG | LEU | GLN | ||||
| 11 | ARG | ALA | ALA |
Samples:
sample_1: ASC2, [U-100% N15; U-100% C13], 0.8 mM
sample_cond_1: ionic strength: 0.8 M; pH: 7.3; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| watergate/presat H1-H1 NOESY | not available | not available | not available |
| H1-H1 presat TOCSY | not available | not available | not available |
| z-filtered DIPSI-TOCSY | not available | not available | not available |
| N15-HSQC | not available | not available | not available |
| N15-TOCSYHSQC | not available | not available | not available |
| N15-NOESYHSQC | not available | not available | not available |
| C13-NOESYHSQC | not available | not available | not available |
| N15,C13-NOESYHSQC | not available | not available | not available |
| C13-HSQC | not available | not available | not available |
| C(CO)NH/C(CC-TOCSY-CO)N-NH | not available | not available | not available |
| H(CCO)NH/H(CC-TOCSY-CO)N-NH | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
Software:
VNMR v6.1C - collection
FELIX v2000 - processing, spectral display
NMR spectrometers:
- Varian UNITYplus 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAG36211 |
| GenBank | AAI05034 AAI05036 AAL58439 AAN03745 AAO23114 |
| REF | NP_690865 XP_001158748 |
| SWISS-PROT | Q8WXC3 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks