BMRB Entry 52326

Name: An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis
Published: 2024-06-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52326

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis

Deposition date:

2024-02-16

Original release date:

2024-06-25

Authors:

Naudi-Fabra, Samuel; Elena-Real, Carlos; Vedel, Ida; Tengo, Maud; Motzny, Kathrin; Jiang, Pin-Lian; Schmieder, Peter; Liu, Fan; Milles, Sigrid

Citation:

Citation: Naudi-Fabra, Samuel; Elena-Real, Carlos; Vedel, Ida; Tengo, Maud; Motzny, Kathrin; Jiang, Pin-Lian; Schmieder, Peter; Liu, Fan; Milles, Sigrid. "An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis" Nat. Commun. 15, 5884-5884 (2024).
PubMed: 39003270

Assembly members:

Assembly members:
entity_1, polymer, 179 residues, 17861.56 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LLMPTMAPSGQPAPVSMVPP SPAMSASKGLGSDLDSSLAS LVGNLGISGTTSKKGDLQWN AGEKKLTGGANWQPKVTPAT WSAGVPPQGTVPPTSSVPPG AGAPSVGQPGAGYGMPPAGT GMTMMPQQPVMFAQPMMRPP FGAAAVPGTQLSPSPTPATQ SPKKPPAKDPLADLNIKDF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	390
15N chemical shifts	119
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AP180 720-898	1

Entities:

Entity 1, AP180 720-898 179 residues - 17861.56 Da.

1

LEU

MET

PRO

THR

MET

ALA

PRO

SER

GLY

2

GLN

PRO

ALA

PRO

VAL

SER

MET

VAL

PRO

3

SER

PRO

ALA

MET

SER

ALA

SER

LYS

GLY

LEU

4

GLY

SER

ASP

LEU

ASP

SER

LEU

ALA

SER

5

LEU

VAL

GLY

ASN

LEU

GLY

ILE

SER

GLY

THR

6

THR

SER

LYS

GLY

ASP

LEU

GLN

TRP

ASN

7

ALA

GLY

GLU

LYS

LEU

THR

GLY

ALA

8

ASN

TRP

GLN

PRO

LYS

VAL

THR

PRO

ALA

THR

9

TRP

SER

ALA

GLY

VAL

PRO

GLN

GLY

THR

10

VAL

PRO

THR

SER

VAL

PRO

GLY

11

ALA

GLY

ALA

PRO

SER

VAL

GLY

GLN

PRO

GLY

12

ALA

GLY

TYR

GLY

MET

PRO

ALA

GLY

THR

13

GLY

MET

THR

MET

PRO

GLN

PRO

VAL

14

MET

PHE

ALA

GLN

PRO

MET

ARG

PRO

15

PHE

GLY

ALA

VAL

PRO

GLY

THR

GLN

16

LEU

SER

PRO

SER

PRO

THR

PRO

ALA

THR

GLN

17

SER

PRO

LYS

PRO

ALA

LYS

ASP

PRO

18

LEU

ALA

ASP

LEU

ASN

ILE

LYS

ASP

PHE

Samples:

sample_1: Monomeric Clathrin Assembly Protein AP180, residues 720-898, [U-100% 13C; U-100% 15N], 100 uM; Na-phosphate 50 mM; NaCl 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0.375 M; pH: 6; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D iHNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D iHNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

MARS - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks