BMRB Entry 52324

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52324

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Title:
An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis
Deposition date:
2024-02-16
Original release date:
2024-06-25
Authors:
Naudi-Fabra, Samuel; Elena-Real, Carlos; Vedel, Ida; Tengo, Maud; Motzny, Kathrin; Jiang, Pin-Lian; Schmieder, Peter; Liu, Fan; Milles, Sigrid
Citation:

Citation: Naudi-Fabra, Samuel; Elena-Real, Carlos; Vedel, Ida; Tengo, Maud; Motzny, Kathrin; Jiang, Pin-Lian; Schmieder, Peter; Liu, Fan; Milles, Sigrid. "An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis"  Nat. Commun. 15, 5884-5884 (2024).
PubMed: 39003270

Assembly members:

Assembly members:
entity_1, polymer, 233 residues, 22135.24 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ACSGNDPFAPSEGSAEAAPE LDLFAMKPPETSAPVVTPTA STAPPVPATAPSPAPTAVAA TAAATTTTTAAAATTTATTS AAAAATAAAPPALDIFGDLF DSAPEVAAASKPDVAPSIDL FGTDAFSSPPRGASPVPESS LTADLLSVDAFAAPSPASTA SPAKAESSGVIDLFGDAFGS SASETQPAPQAVSSSSASAD LLAGFGGSFMAPSTTPVTPA QNNLLQPNFEAAL

Data sets:
Data typeCount
13C chemical shifts603
15N chemical shifts176
1H chemical shifts177

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AP180 471-7001

Entities:

Entity 1, AP180 471-700 233 residues - 22135.24 Da.

1   ALACYSSERGLYASNASPPROPHEALAPRO
2   SERGLUGLYSERALAGLUALAALAPROGLU
3   LEUASPLEUPHEALAMETLYSPROPROGLU
4   THRSERALAPROVALVALTHRPROTHRALA
5   SERTHRALAPROPROVALPROALATHRALA
6   PROSERPROALAPROTHRALAVALALAALA
7   THRALAALAALATHRTHRTHRTHRTHRALA
8   ALAALAALATHRTHRTHRALATHRTHRSER
9   ALAALAALAALAALATHRALAALAALAPRO
10   PROALALEUASPILEPHEGLYASPLEUPHE
11   ASPSERALAPROGLUVALALAALAALASER
12   LYSPROASPVALALAPROSERILEASPLEU
13   PHEGLYTHRASPALAPHESERSERPROPRO
14   ARGGLYALASERPROVALPROGLUSERSER
15   LEUTHRALAASPLEULEUSERVALASPALA
16   PHEALAALAPROSERPROALASERTHRALA
17   SERPROALALYSALAGLUSERSERGLYVAL
18   ILEASPLEUPHEGLYASPALAPHEGLYSER
19   SERALASERGLUTHRGLNPROALAPROGLN
20   ALAVALSERSERSERSERALASERALAASP
21   LEULEUALAGLYPHEGLYGLYSERPHEMET
22   ALAPROSERTHRTHRPROVALTHRPROALA
23   GLNASNASNLEULEUGLNPROASNPHEGLU
24   ALAALALEU

Samples:

sample_1: Monomeric Clathrin Assembly Protein AP180, residues 471-700, [U-100% 13C; U-100% 15N], 100 uM; Na-phosphate 50 mM; NaCl 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0.375 M; pH: 6; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D iHNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D iHNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

MARS - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks