BMRB Entry 52320

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52320

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Title:
An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis
Deposition date:
2024-02-13
Original release date:
2024-06-25
Authors:
Naudi-Fabra, Samuel; Elena-Real, Carlos; Vedel, Ida Marie; Tengo, Maud; Motzny, Kathrin; Jiang, Pin-Lian; Schmieder, Peter; Liu, Fan; Papagiannoula, Andromachi; Milles, Sigrid
Citation:

Citation: Naudi-Fabra, Samuel; Elena-Real, Carlos; Vedel, Ida Marie; Tengo, Maud; Motzny, Kathrin; Jiang, Pin-Lian; Schmieder, Peter; Liu, Fan; Milles, Sigrid. "An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis"  Nat. Commun. 15, 5884-5884 (2024).
PubMed: 39003270

Assembly members:

Assembly members:
entity_1, polymer, 252 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMIGMAPGGYVAPKAVWLP AVKAKGLEISGTFTHRQGHI YMEMNFTNKALQHMTDFAIQ FNKNSFGVIPSTPLAIHTPL MPNQSIDVSLPLNTLGPVMK MEPLNNLQVAVKNNIDVFYF SCLIPLNVLFVEDGKMERQV FLATWKDIPNENELQFQIKE CHLNADTVSSKLQNNNVYTI AKRNVEGQDMLYQSLKLTNG IWILAELRIQPGNPNYTLSL KCRAPEVSQYIYQVYDSILK NAAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts594
15N chemical shifts170
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AP2B21

Entities:

Entity 1, AP2B2 252 residues - Formula weight is not available

1   GLYHISMETILEGLYMETALAPROGLYGLY
2   TYRVALALAPROLYSALAVALTRPLEUPRO
3   ALAVALLYSALALYSGLYLEUGLUILESER
4   GLYTHRPHETHRHISARGGLNGLYHISILE
5   TYRMETGLUMETASNPHETHRASNLYSALA
6   LEUGLNHISMETTHRASPPHEALAILEGLN
7   PHEASNLYSASNSERPHEGLYVALILEPRO
8   SERTHRPROLEUALAILEHISTHRPROLEU
9   METPROASNGLNSERILEASPVALSERLEU
10   PROLEUASNTHRLEUGLYPROVALMETLYS
11   METGLUPROLEUASNASNLEUGLNVALALA
12   VALLYSASNASNILEASPVALPHETYRPHE
13   SERCYSLEUILEPROLEUASNVALLEUPHE
14   VALGLUASPGLYLYSMETGLUARGGLNVAL
15   PHELEUALATHRTRPLYSASPILEPROASN
16   GLUASNGLULEUGLNPHEGLNILELYSGLU
17   CYSHISLEUASNALAASPTHRVALSERSER
18   LYSLEUGLNASNASNASNVALTYRTHRILE
19   ALALYSARGASNVALGLUGLYGLNASPMET
20   LEUTYRGLNSERLEULYSLEUTHRASNGLY
21   ILETRPILELEUALAGLULEUARGILEGLN
22   PROGLYASNPROASNTYRTHRLEUSERLEU
23   LYSCYSARGALAPROGLUVALSERGLNTYR
24   ILETYRGLNVALTYRASPSERILELEULYS
25   ASNALAALAALALEUGLUHISHISHISHIS
26   HISHIS

Samples:

sample_1: AP2B2, [U-13C; U-15N; U-2H], 420 uM; Na-phosphate 50 mM; NaCl 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0.375 M; pH: 6; pressure: 1 atm; temperature: 298.15 K

sample_conditions_2: ionic strength: 0.375 M; pH: 6; pressure: 1 atm; temperature: 305.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N BEST-TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D iHNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D iHNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

MARS - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks