BMRB Entry 5232
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5232
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Mitton-Fry, Rachel; Wuttke, Deborah. "Letter to the Editor: 1H, 13C, and 15N resonance assignments of the DNA-binding
domain of the essential protein Cdc13 complexed with single-stranded telomeric
DNA " J. Biomol. NMR 22, 379-380 (2002).
PubMed: 12018489
Assembly members:
Cdc13 DNA-binding domain, polymer, 199 residues, Formula weight is not available
telomeric single-stranded DNA 11mer, polymer, 11 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a
Entity Sequences (FASTA):
Cdc13 DNA-binding domain: MRMSKMARKDPTIEFCQLGL
DTFETKYITMFGMLVSCSFD
KPAFISFVFSDFTKNDIVQN
YLYDRYLIDYENKLELNEGF
KAIMYKNQFETFDSKLRKIF
NNGLRDLQNGRDENLSQYGI
VCKMNIKVKMYNGKLNAIVR
ECEPVPHSQISSIASPSQCE
HLRLFYQRAFKRIGESAISR
YFEEYRRFFPIHRNGSHLA
telomeric single-stranded DNA 11mer: GTGTGGGTGTG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1238 |
| 13C chemical shifts | 823 |
| 15N chemical shifts | 184 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cdc13 DNA-binding Domain | 1 |
| 2 | telomeric single-stranded DNA | 2 |
Entities:
Entity 1, Cdc13 DNA-binding Domain 199 residues - Formula weight is not available
| 1 | MET | ARG | MET | SER | LYS | MET | ALA | ARG | LYS | ASP | ||||
| 2 | PRO | THR | ILE | GLU | PHE | CYS | GLN | LEU | GLY | LEU | ||||
| 3 | ASP | THR | PHE | GLU | THR | LYS | TYR | ILE | THR | MET | ||||
| 4 | PHE | GLY | MET | LEU | VAL | SER | CYS | SER | PHE | ASP | ||||
| 5 | LYS | PRO | ALA | PHE | ILE | SER | PHE | VAL | PHE | SER | ||||
| 6 | ASP | PHE | THR | LYS | ASN | ASP | ILE | VAL | GLN | ASN | ||||
| 7 | TYR | LEU | TYR | ASP | ARG | TYR | LEU | ILE | ASP | TYR | ||||
| 8 | GLU | ASN | LYS | LEU | GLU | LEU | ASN | GLU | GLY | PHE | ||||
| 9 | LYS | ALA | ILE | MET | TYR | LYS | ASN | GLN | PHE | GLU | ||||
| 10 | THR | PHE | ASP | SER | LYS | LEU | ARG | LYS | ILE | PHE | ||||
| 11 | ASN | ASN | GLY | LEU | ARG | ASP | LEU | GLN | ASN | GLY | ||||
| 12 | ARG | ASP | GLU | ASN | LEU | SER | GLN | TYR | GLY | ILE | ||||
| 13 | VAL | CYS | LYS | MET | ASN | ILE | LYS | VAL | LYS | MET | ||||
| 14 | TYR | ASN | GLY | LYS | LEU | ASN | ALA | ILE | VAL | ARG | ||||
| 15 | GLU | CYS | GLU | PRO | VAL | PRO | HIS | SER | GLN | ILE | ||||
| 16 | SER | SER | ILE | ALA | SER | PRO | SER | GLN | CYS | GLU | ||||
| 17 | HIS | LEU | ARG | LEU | PHE | TYR | GLN | ARG | ALA | PHE | ||||
| 18 | LYS | ARG | ILE | GLY | GLU | SER | ALA | ILE | SER | ARG | ||||
| 19 | TYR | PHE | GLU | GLU | TYR | ARG | ARG | PHE | PHE | PRO | ||||
| 20 | ILE | HIS | ARG | ASN | GLY | SER | HIS | LEU | ALA |
Entity 2, telomeric single-stranded DNA 11 residues - Formula weight is not available
| 1 | DG | DT | DG | DT | DG | DG | DG | DT | DG | DT | ||||
| 2 | DG |
Samples:
sample_1: Cdc13 DNA-binding domain, [U-13C; U-15N], 0.7 – 1.1 mM; telomeric single-stranded DNA 11mer0.8 – 1.2 mM; imidazole, [U-2H], 50 mM; sodium chloride 150 mM; sodium sulfate 100 mM; sodium azide 0.02%; DTT, [U-2H], 2 mM
sample_2: Cdc13 DNA-binding domain, [U-15N], 1.5 mM; telomeric single-stranded DNA 11mer 1.7 mM; imidazole, [U-2H], 50 mM; sodium chloride 150 mM; sodium sulfate 100 mM; sodium azide 0.02%; DTT, [U-2H], 2 mM
sample_3: Cdc13 DNA-binding domain 1.1 mM; telomeric single-stranded DNA 11mer 1.2 mM; imidazole, [U-2H], 50 mM; sodium chloride 150 mM; sodium sulfate 100 mM; sodium azide 0.02%; DTT, [U-2H], 2 mM
Ex-cond_1: pH: 7.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-13C CT-HSQC | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 2D CBHD | not available | not available | not available |
| 2D CBHE | not available | not available | not available |
| 3D 1H-13C-1H HCCH-COSY | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 13C-1H-1H NOESY | not available | not available | not available |
| 3D 1H-13C-1H HCCH-TOCSY | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D HCACO | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D HN(CA)CO | not available | not available | not available |
| 3D C(CO)NH | not available | not available | not available |
| 3D HC(CO)NH | not available | not available | not available |
| 4D 1H-15N-1H-13C NOESY | not available | not available | not available |
| 4D 1H-13C-13C-1H NOESY | not available | not available | not available |
Software:
NMRPipe - data processing
Ansig v3.3 - data analysis
NMR spectrometers:
- Varian UnityInova 500 MHz
- Varian UnityInova 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB | |
| DBJ | GAA22033 |
| EMBL | CAA98800 CAY79065 |
| GB | AAA99990 AHY74797 EDN60143 EDV08479 EDZ73439 |
| REF | NP_010061 |
| SP | P32797 |
| TPG | DAA11644 |
| AlphaFold | P32797 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks