BMRB Entry 52304

Name: Backbone chemical shifts for the human Fc gamma receptor 3a / CD16a soluble extracellular domain that is glycosylated with two N-glycans at N162 and N45
Published: 2024-08-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52304

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shifts for the human Fc gamma receptor 3a / CD16a soluble extracellular domain that is glycosylated with two N-glycans at N162 and N45

Deposition date:

2024-02-02

Original release date:

2024-08-16

Authors:

Barb, Adam

Citation:

Citation: Barb, Adam. "Backbone chemical shifts for the human Fc gamma receptor 3a / CD16a soluble extracellular domain that is glycosylated with two N-glycans at N162 and N45" .

Assembly members:

Assembly members:
entity_1, polymer, 175 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Homo sapiens Vector: pGen2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RTEDLPKAVVFLEPQWYRVL EKDSVTLKCQGAYSPEDQST QWFHNESLISSQASSYFIDA ATVDDSGEYRCQTQLSTLSD PVQLEVHIGWLLLQAPRWVF KEEDPIHLRCHSWKNTALHK VTYLQNGKGRKYFHHNSDFY IPKATLKDSGSYFCRGLVGS KNVSSETVQITITQG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	293
15N chemical shifts	149
1H chemical shifts	149

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD16a	1

Entities:

Entity 1, CD16a 175 residues - Formula weight is not available

1

ARG

THR

GLU

ASP

LEU

PRO

LYS

ALA

VAL

2

PHE

LEU

GLU

PRO

GLN

TRP

TYR

ARG

VAL

LEU

3

GLU

LYS

ASP

SER

VAL

THR

LEU

LYS

CYS

GLN

4

GLY

ALA

TYR

SER

PRO

GLU

ASP

GLN

SER

THR

5

GLN

TRP

PHE

HIS

ASN

GLU

SER

LEU

ILE

SER

6

SER

GLN

ALA

SER

TYR

PHE

ILE

ASP

ALA

7

ALA

THR

VAL

ASP

SER

GLY

GLU

TYR

ARG

8

CYS

GLN

THR

GLN

LEU

SER

THR

LEU

SER

ASP

9

PRO

VAL

GLN

LEU

GLU

VAL

HIS

ILE

GLY

TRP

10

LEU

GLN

ALA

PRO

ARG

TRP

VAL

PHE

11

LYS

GLU

ASP

PRO

ILE

HIS

LEU

ARG

CYS

12

HIS

SER

TRP

LYS

ASN

THR

ALA

LEU

HIS

LYS

13

VAL

THR

TYR

LEU

GLN

ASN

GLY

LYS

GLY

ARG

14

LYS

TYR

PHE

HIS

ASN

SER

ASP

PHE

TYR

15

ILE

PRO

LYS

ALA

THR

LEU

LYS

ASP

SER

GLY

16

SER

TYR

PHE

CYS

ARG

GLY

LEU

VAL

GLY

SER

17

LYS

ASN

VAL

SER

GLU

THR

VAL

GLN

ILE

18

THR

ILE

THR

GLN

GLY

Samples:

sample_1: Fc gamma receptor 3a, V158 allotrope, [U-95% 13C; U-90% 15N], 200 uM; MOPS 25 mM; KCl 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

VNMRj - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks