BMRB Entry 52292

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52292

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Title:
NMR assignment CDCA3 WT
Deposition date:
2024-01-30
Original release date:
2024-03-25
Authors:
Boll, Emmanuelle; Cantrelle, Francois-Xavier; Bridot, Clarisse; Adams, Mark; Smet-Nocca, Caroline
Citation:

Citation: Boll, Emmanuelle; Cantrelle, Francois-Xavier; Bridot, Clarisse; Adams, Mark; Smet-Nocca, Caroline. "NMR assignment CDCA3 WT"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
entity_1, polymer, 299 residues, 32238.6454 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GLNDIFEAQKIEWHEGSAGG SGGSAKSVPVTPARPPPHNK HLARVADPRSPSAGILRTPI QVESSPQPGLPAGEQLEGLK HAQDSDPRSPTLGIARTPMK TSSGDPPSPLVKQLSEVFET EDSKSNLPPEPVLPPEAPLS SELDLPLGTQLSVEEQMPPW NQTEFPSKQVFSKEEARQPT ETPVASQSSDKPSRDPETPR SSGSMRNRWKPNSSKVLGRS PLTILQDDNSPGTLTLRQGK RPSPLSENVSELKEGAILGT GRLLKTGGRAWEQGQDHDKE NQHFPLVESGGSGHHHHHH

Data sets:
Data typeCount
13C chemical shifts743
15N chemical shifts242
1H chemical shifts445

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CDCA31

Entities:

Entity 1, CDCA3 299 residues - 32238.6454 Da.

1   GLYLEUASNASPILEPHEGLUALAGLNLYS
2   ILEGLUTRPHISGLUGLYSERALAGLYGLY
3   SERGLYGLYSERALALYSSERVALPROVAL
4   THRPROALAARGPROPROPROHISASNLYS
5   HISLEUALAARGVALALAASPPROARGSER
6   PROSERALAGLYILELEUARGTHRPROILE
7   GLNVALGLUSERSERPROGLNPROGLYLEU
8   PROALAGLYGLUGLNLEUGLUGLYLEULYS
9   HISALAGLNASPSERASPPROARGSERPRO
10   THRLEUGLYILEALAARGTHRPROMETLYS
11   THRSERSERGLYASPPROPROSERPROLEU
12   VALLYSGLNLEUSERGLUVALPHEGLUTHR
13   GLUASPSERLYSSERASNLEUPROPROGLU
14   PROVALLEUPROPROGLUALAPROLEUSER
15   SERGLULEUASPLEUPROLEUGLYTHRGLN
16   LEUSERVALGLUGLUGLNMETPROPROTRP
17   ASNGLNTHRGLUPHEPROSERLYSGLNVAL
18   PHESERLYSGLUGLUALAARGGLNPROTHR
19   GLUTHRPROVALALASERGLNSERSERASP
20   LYSPROSERARGASPPROGLUTHRPROARG
21   SERSERGLYSERMETARGASNARGTRPLYS
22   PROASNSERSERLYSVALLEUGLYARGSER
23   PROLEUTHRILELEUGLNASPASPASNSER
24   PROGLYTHRLEUTHRLEUARGGLNGLYLYS
25   ARGPROSERPROLEUSERGLUASNVALSER
26   GLULEULYSGLUGLYALAILELEUGLYTHR
27   GLYARGLEULEULYSTHRGLYGLYARGALA
28   TRPGLUGLNGLYGLNASPHISASPLYSGLU
29   ASNGLNHISPHEPROLEUVALGLUSERGLY
30   GLYSERGLYHISHISHISHISHISHIS

Samples:

sample_1: CDCA3_WT, [U-100% 13C; U-100% 15N], 350 uM; DTT 1 mM; sodium chloride 50 mM; EDTA 2.5 mM; sodium phosphate 25 mM

sample_2: CDCA3_WT, [U-100% 13C; U-100% 15N], 184 uM; DTT 1 mM; sodium chloride 50 mM; EDTA 2.5 mM; sodium phosphate 25 mM

sample_conditions_1: ionic strength: 0,092 M; pH: 6.6; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_2isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
CO[N]sample_1isotropicsample_conditions_1
hNcocaNNHsample_2isotropicsample_conditions_1
HncocaNHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1

Software:

TOPSPIN v4.1.3 - collection

CcpNMR v2.5 - peak picking

qMDD v2.6 - processing

NMR spectrometers:

  • Bruker AVANCE NEO 900 MHz
  • Bruker AVANCE NEO 1200 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks