BMRB Entry 52290

Name: 1H,15N and 13C backbone chemical shift assignment of NanogH
Published: 2025-12-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52290

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H,15N and 13C backbone chemical shift assignment of NanogH

Deposition date:

2024-01-29

Original release date:

2025-12-20

Authors:

Xue, HongJuan; Wu, Bin; Wang, Duo; Li, Yanjing; Chen, Yong

Citation:

Citation: Wang, Duo; Li, Yanjing; Chen, Yong. "WDR5 Reshapes Nanog Prion-like Structure to Sustain Pluripotency in Embryonic Stem Cells" .

Assembly members:

Assembly members:
entity_1, polymer, 60 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KQKMRTVFSQAQLCALKDRF QKQKYLSLQQMQELSSILNL SYKQVKTWFQNQRMKCKRWQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	180
15N chemical shifts	61
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NanogH	1

Entities:

Entity 1, NanogH 60 residues - Formula weight is not available

1	LYS	GLN	LYS	MET	ARG	THR	VAL	PHE	SER	GLN
2	ALA	GLN	LEU	CYS	ALA	LEU	LYS	ASP	ARG	PHE
3	GLN	LYS	GLN	LYS	TYR	LEU	SER	LEU	GLN	GLN
4	MET	GLN	GLU	LEU	SER	SER	ILE	LEU	ASN	LEU
5	SER	TYR	LYS	GLN	VAL	LYS	THR	TRP	PHE	GLN
6	ASN	GLN	ARG	MET	LYS	CYS	LYS	ARG	TRP	GLN

Samples:

sample_1: NanogH, [U-100% 13C; U-100% 15N], 0.5 mM; HEPS 15 mM; NaCl 300 mM; DTT 3 mM; NaN3 0.03%

sample_conditions_1: ionic strength: 0.3 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

PINE - chemical shift assignment

NMRFAM-SPARKY - data analysis

NMR spectrometers:

Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	LYS	GLN	LYS	MET	ARG	THR	VAL	PHE	SER	GLN
2	ALA	GLN	LEU	CYS	ALA	LEU	LYS	ASP	ARG	PHE
3	GLN	LYS	GLN	LYS	TYR	LEU	SER	LEU	GLN	GLN
4	MET	GLN	GLU	LEU	SER	SER	ILE	LEU	ASN	LEU
5	SER	TYR	LYS	GLN	VAL	LYS	THR	TRP	PHE	GLN
6	ASN	GLN	ARG	MET	LYS	CYS	LYS	ARG	TRP	GLN

1	LYS	GLN	LYS	MET	ARG	THR	VAL	PHE	SER	GLN
2	ALA	GLN	LEU	CYS	ALA	LEU	LYS	ASP	ARG	PHE
3	GLN	LYS	GLN	LYS	TYR	LEU	SER	LEU	GLN	GLN
4	MET	GLN	GLU	LEU	SER	SER	ILE	LEU	ASN	LEU
5	SER	TYR	LYS	GLN	VAL	LYS	THR	TRP	PHE	GLN
6	ASN	GLN	ARG	MET	LYS	CYS	LYS	ARG	TRP	GLN

1	LYS	GLN	LYS	MET	ARG	THR	VAL	PHE	SER	GLN
2	ALA	GLN	LEU	CYS	ALA	LEU	LYS	ASP	ARG	PHE
3	GLN	LYS	GLN	LYS	TYR	LEU	SER	LEU	GLN	GLN
4	MET	GLN	GLU	LEU	SER	SER	ILE	LEU	ASN	LEU
5	SER	TYR	LYS	GLN	VAL	LYS	THR	TRP	PHE	GLN
6	ASN	GLN	ARG	MET	LYS	CYS	LYS	ARG	TRP	GLN