BMRB Entry 52285

Name: pre-miR-20a
Published: 2025-07-07

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PDB ID: 9obm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52285
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

pre-miR-20a

Deposition date:

2024-01-24

Original release date:

2025-07-07

Authors:

Liu, Yaping; Harkner, Cade; Munsayac, Aldrex; Keane, Sarah

Citation:

Citation: Liu, Yaping; Harkner, Cade; Westwood, Megan; Munsayac, Aldrex; Keane, Sarah. "Structural features within precursor microRNA-20a regulate Dicer-TRBP processing" J. Mol. Biol. ., .-. (2025).
PubMed: 40609633

Assembly members:

Assembly members:
entity_1, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: In vitro transcription Host organism: In vitro

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGUAGCACUAAAGUGCUUAU AGUGCAGGUAGUGUUUAGUU AUCUACUGCAUUAUGAGCAC UUAAAGUACUGCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	81
15N chemical shifts	33
1H chemical shifts	348

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pre-miR-20a	1

Entities:

Entity 1, pre-miR-20a 73 residues - Formula weight is not available

1

G

U

A

G

C

A

C

U

A

2

A

G

U

G

C

U

A

U

3

A

G

U

G

C

A

G

U

A

4

G

U

G

U

A

G

U

5

A

U

C

U

A

C

U

G

C

A

6

U

A

U

G

A

G

C

A

C

7

U

A

G

U

A

C

U

8

G

C

Samples:

sample_1: pre-miR-20a 600 uM; potassium phosphate 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC/HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRFx Analyst - processing

NMRViewJ - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz