BMRB Entry 52284

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52284

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Title:
Efg1 C-PrLD assignments
Deposition date:
2024-01-24
Original release date:
2024-02-13
Authors:
Zheng, Tongyin; Wang, Szu-Huan; Fawzi, Nicolas
Citation:

Citation: Wang, Szu-Huan; Zheng, Tongyin; Fawzi, Nicolas. "Structure and interactions of prion-like domains in transcription factor Efg1 phase separation"  Biophys. J. 123, 1481-1493 (2024).
PubMed: 38297837

Assembly members:

Assembly members:
entity_1, polymer, 201 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: budding yeasts   Taxonomy ID: 5476   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Candida albicans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMSGNGNSISATSGGSNVS GASGAGSTTSPVNTKAATAA GIPQGNYYQTYNQQQYPQQY GQYNAPGKNQNTPASQPGST TNDQYLQQQQQQQQQMYGYQ SNYYQGGAANSSYYPNYYQQ QQPNYASSYPYQQQQQKQQQ QQPNQQQQSDQQQTSTPSGG AGTRSVHQSPQVQSLTQGSV HPSPQQHQANQSASTVAKEE K

Data sets:
Data typeCount
13C chemical shifts566
15N chemical shifts179
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Efg1 C-PrLD1

Entities:

Entity 1, Efg1 C-PrLD 201 residues - Formula weight is not available

1   GLYHISMETSERGLYASNGLYASNSERILE
2   SERALATHRSERGLYGLYSERASNVALSER
3   GLYALASERGLYALAGLYSERTHRTHRSER
4   PROVALASNTHRLYSALAALATHRALAALA
5   GLYILEPROGLNGLYASNTYRTYRGLNTHR
6   TYRASNGLNGLNGLNTYRPROGLNGLNTYR
7   GLYGLNTYRASNALAPROGLYLYSASNGLN
8   ASNTHRPROALASERGLNPROGLYSERTHR
9   THRASNASPGLNTYRLEUGLNGLNGLNGLN
10   GLNGLNGLNGLNGLNMETTYRGLYTYRGLN
11   SERASNTYRTYRGLNGLYGLYALAALAASN
12   SERSERTYRTYRPROASNTYRTYRGLNGLN
13   GLNGLNPROASNTYRALASERSERTYRPRO
14   TYRGLNGLNGLNGLNGLNLYSGLNGLNGLN
15   GLNGLNPROASNGLNGLNGLNGLNSERASP
16   GLNGLNGLNTHRSERTHRPROSERGLYGLY
17   ALAGLYTHRARGSERVALHISGLNSERPRO
18   GLNVALGLNSERLEUTHRGLNGLYSERVAL
19   HISPROSERPROGLNGLNHISGLNALAASN
20   GLNSERALASERTHRVALALALYSGLUGLU
21   LYS

Samples:

sample_1: Efg1 C-PrLD, [U-100% 13C; U-100% 15N], 100 uM; MES 20 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - data analysis

NMRPipe - processing

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks