BMRB Entry 52282

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52282

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Title:
Backbone resonances of S. pombe Puf3 (residue 1-258)
Deposition date:
2024-01-24
Original release date:
2025-09-03
Authors:
Yu, Conny; Stowell, James; Freund, Stefan; Passmore, Lori
Citation:

Citation: Yu, Conny; Stowell, James; Freund, Stefan; Passmore, Lori. "Multivalent interactions between RNA-binding proteins and CCR4-NOT enable phosphorylation-dependent tuning of mRNA deadenylation rates"  .

Assembly members:

Assembly members:
entity_1, polymer, 269 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPHMFTAVNSNPNASESISG NSAFNFPSAPVSSLDTNNYG QRRPSLLSGTSPTSSFFNSS MISSNYTFPHGSNKQASLES PVSYSNPIPSLTWLSLDGDS PDSLVSTPTAPSANHHGNPF PNGKQSIKAMPSLVNLQEDS VISKFPNSLEVPFRKRSEST SSSLSGLHSDLRPLKTELYG QLNSECGARFPQTLKSPLTP IGGDSARTVSASTARTSDKF FPRHTRAHSDFWIPATSKPS RHASHSSIGDLTTITQSSIS SSGLEVLFQ

Data sets:
Data typeCount
13C chemical shifts755
15N chemical shifts265
1H chemical shifts222

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Puf3_1-2581

Entities:

Entity 1, Puf3_1-258 269 residues - Formula weight is not available

1   GLYPROHISMETPHETHRALAVALASNSER
2   ASNPROASNALASERGLUSERILESERGLY
3   ASNSERALAPHEASNPHEPROSERALAPRO
4   VALSERSERLEUASPTHRASNASNTYRGLY
5   GLNARGARGPROSERLEULEUSERGLYTHR
6   SERPROTHRSERSERPHEPHEASNSERSER
7   METILESERSERASNTYRTHRPHEPROHIS
8   GLYSERASNLYSGLNALASERLEUGLUSER
9   PROVALSERTYRSERASNPROILEPROSER
10   LEUTHRTRPLEUSERLEUASPGLYASPSER
11   PROASPSERLEUVALSERTHRPROTHRALA
12   PROSERALAASNHISHISGLYASNPROPHE
13   PROASNGLYLYSGLNSERILELYSALAMET
14   PROSERLEUVALASNLEUGLNGLUASPSER
15   VALILESERLYSPHEPROASNSERLEUGLU
16   VALPROPHEARGLYSARGSERGLUSERTHR
17   SERSERSERLEUSERGLYLEUHISSERASP
18   LEUARGPROLEULYSTHRGLULEUTYRGLY
19   GLNLEUASNSERGLUCYSGLYALAARGPHE
20   PROGLNTHRLEULYSSERPROLEUTHRPRO
21   ILEGLYGLYASPSERALAARGTHRVALSER
22   ALASERTHRALAARGTHRSERASPLYSPHE
23   PHEPROARGHISTHRARGALAHISSERASP
24   PHETRPILEPROALATHRSERLYSPROSER
25   ARGHISALASERHISSERSERILEGLYASP
26   LEUTHRTHRILETHRGLNSERSERILESER
27   SERSERGLYLEUGLUVALLEUPHEGLN

Samples:

sample_1: Puf3_1-258, [U-100% 15N], 100 uM; PIPES 20 mM; sodium chloride 50 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, data analysis

NMRFAM-SPARKY - data analysis

POKY - data analysis

MARS - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks