BMRB Entry 52270

Name: pfUCHL3
Published: 2024-04-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52270

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

pfUCHL3

Deposition date:

2024-01-15

Original release date:

2024-04-30

Authors:

Bycroft, Mark

Citation:

Citation: King, Harry; Bycroft, Mark; Nguyen, Thanh-Binh; Kelly, Geoff; Vinogradov, Alexander; Rowling, Pamela; Stott, Katherine; Ascher, David; Suga, Hiroaki; Itzhaki, Laura; Artavanis-Tsakonas, Katerina. "Targeting the Plasmodium falciparum UCHL3 ubiquitin hydrolase using chemically constrained peptides" Proc. Natl. Acad. Sci. U.S.A. 121, e2322923121-e2322923121 (2024).
PubMed: 38739798

Assembly members:

Assembly members:
entity_1, polymer, 235 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Plasmodium falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMAKNDIWTPLESNPDSL YLYSCKLGQSKLKFVDIYGF NNDLLDMIPQPVQAVIFLYP VNDNIVSENNTNDKHNLKEN FDNVWFIKQYIPNSCGTIAL LHLYGNLRNKFELDKDSVLD DFFNKVNEMSAEKRGQELKN NKSIENLHHEFCGQVENRDD ILDVDTHFIVFVQIEGKIIE LDGRKDHPTVHCFTNGDNFL YDTGKIIQDKFIEKCKDDLR FSALAVIPNDNFDII

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	566
15N chemical shifts	209
1H chemical shifts	209

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pfUCHl3	1

Entities:

Entity 1, pfUCHl3 235 residues - Formula weight is not available

1

GLY

SER

HIS

MET

ALA

LYS

ASN

ASP

ILE

TRP

2

THR

PRO

LEU

GLU

SER

ASN

PRO

ASP

SER

LEU

3

TYR

LEU

TYR

SER

CYS

LYS

LEU

GLY

GLN

SER

4

LYS

LEU

LYS

PHE

VAL

ASP

ILE

TYR

GLY

PHE

5

ASN

ASP

LEU

ASP

MET

ILE

PRO

GLN

6

PRO

VAL

GLN

ALA

VAL

ILE

PHE

LEU

TYR

PRO

7

VAL

ASN

ASP

ASN

ILE

VAL

SER

GLU

ASN

8

THR

ASN

ASP

LYS

HIS

ASN

LEU

LYS

GLU

ASN

9

PHE

ASP

ASN

VAL

TRP

PHE

ILE

LYS

GLN

TYR

10

ILE

PRO

ASN

SER

CYS

GLY

THR

ILE

ALA

LEU

11

LEU

HIS

LEU

TYR

GLY

ASN

LEU

ARG

ASN

LYS

12

PHE

GLU

LEU

ASP

LYS

ASP

SER

VAL

LEU

ASP

13

ASP

PHE

ASN

LYS

VAL

ASN

GLU

MET

SER

14

ALA

GLU

LYS

ARG

GLY

GLN

GLU

LEU

LYS

ASN

15

ASN

LYS

SER

ILE

GLU

ASN

LEU

HIS

GLU

16

PHE

CYS

GLY

GLN

VAL

GLU

ASN

ARG

ASP

17

ILE

LEU

ASP

VAL

ASP

THR

HIS

PHE

ILE

VAL

18

PHE

VAL

GLN

ILE

GLU

GLY

LYS

ILE

GLU

19

LEU

ASP

GLY

ARG

LYS

ASP

HIS

PRO

THR

VAL

20

HIS

CYS

PHE

THR

ASN

GLY

ASP

ASN

PHE

LEU

21

TYR

ASP

THR

GLY

LYS

ILE

GLN

ASP

LYS

22

PHE

ILE

GLU

LYS

CYS

LYS

ASP

LEU

ARG

23

PHE

SER

ALA

LEU

ALA

VAL

ILE

PRO

ASN

ASP

24

ASN

PHE

ASP

ILE

Samples:

sample_1: pfUCHL3, [U-99% 13C; U-99% 15N], 250 uM; potassium phosphate 20 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - processing

NMRFAM-SPARKY - peak picking

MARS - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz