BMRB Entry 52269

Name: MTMR7 coiled coil domain
Published: 2024-03-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52269

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

MTMR7 coiled coil domain

Deposition date:

2024-01-10

Original release date:

2024-03-28

Authors:

Saar, Daniel; Prestel, Andreas; Kragelund, Birthe

Citation:

Citation: Weidner, Philip; Saar, Daniel; Sohn, Michaela; Schroeder, Torsten; Yu, Yanxiong; Zollner, Frank; Ponelies, Norbert; Zhou, Xiaobo; Zwicky, Andre; Rohrbacher, Florian; Pattabiraman, Vijaya; Tanriver, Matthias; Bauer, Alexander; Ahmed, Hazem; Ametamey, Simon; Riffel, Philipp; Seger, Rony; Bode, Jeffrey; Wade, Rebecca; Ebert, Matthias; Kragelund, Birthe; Burgermeister, Elke. "Myotubularin-related-protein-7 inhibits mutant (G12V) K-RAS by direct interaction" Cancer Lett. (Shannon, Irel.) 588, 216783-216783 (2024).
PubMed: 38462034

Assembly members:

Assembly members:
entity_1, polymer, 41 residues, 4981 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RQSWTDYLMAVKEETQQLEE ELEALEERLEKIQKVQLNCT K

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	114
15N chemical shifts	38
1H chemical shifts	37

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MTMR7 monomer	1

Entities:

Entity 1, MTMR7 monomer 41 residues - 4981 Da.

1

ARG

GLN

SER

TRP

THR

ASP

TYR

LEU

MET

ALA

2

VAL

LYS

GLU

THR

GLN

LEU

GLU

3

GLU

LEU

GLU

ALA

LEU

GLU

ARG

LEU

GLU

4

LYS

ILE

GLN

LYS

VAL

GLN

LEU

ASN

CYS

THR

5

LYS

Samples:

sample_1: MTMR7-CC, [U-99% 13C; U-99% 15N], 160 uM; D2O, [U-100% 2H], 10%; DSS 0.7 mM; sodium phosphate 20 mM; sodium chloride 137 mM; DTT 3 mM

sample_conditions_1: ionic strength: 137 mM; pH: 7.3; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR - data analysis

NMR spectrometers:

Bruker AVANCE III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks