BMRB Entry 52266

Name: Backbone chemical shift assignment of HoxA9 protein
Published: 2024-02-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52266

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone chemical shift assignment of HoxA9 protein

Deposition date:

2024-01-09

Original release date:

2024-02-08

Authors:

Saibo, Nikita; De, Soumya

Citation:

Citation: Saibo, Nikita; Maiti, Snigdha; Boral, Soumendu; Banerjee, Puja; Kushwaha, Tushar; Inampudi, Krishna; Goswami, Ritobrata; De, Soumya. "The intrinsically disordered transactivation region of HOXA9 regulates its function by auto-inhibition of its DNA-binding activity" IJBM ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RRRRRRRRNNPAANWLHARS TRKKRCPYTKHQTLELEKEF LFNMYLTRDRRYEVARLLNL TERQVKIWFQNRRMKMKKIN KDRAKDE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	142
15N chemical shifts	75
1H chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HoxA9 Monomer	1

Entities:

Entity 1, HoxA9 Monomer 87 residues - Formula weight is not available

Residue 194-266

1

ARG

ASN

2

PRO

ALA

ASN

TRP

LEU

HIS

ALA

ARG

SER

3

THR

ARG

LYS

ARG

CYS

PRO

TYR

THR

LYS

4

HIS

GLN

THR

LEU

GLU

LEU

GLU

LYS

GLU

PHE

5

LEU

PHE

ASN

MET

TYR

LEU

THR

ARG

ASP

ARG

6

ARG

TYR

GLU

VAL

ALA

ARG

LEU

ASN

LEU

7

THR

GLU

ARG

GLN

VAL

LYS

ILE

TRP

PHE

GLN

8

ASN

ARG

MET

LYS

MET

LYS

ILE

ASN

9

LYS

ASP

ARG

ALA

LYS

ASP

GLU

Samples:

sample_1: HoxA9 Monomer, [U-100% 13C], 0.3 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks