BMRB Entry 52258

Name: Efg1 N prion-like domain assignments
Published: 2024-02-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52258

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Efg1 N prion-like domain assignments

Deposition date:

2024-01-03

Original release date:

2024-02-13

Authors:

Zheng, Tongyin; Wang, Szu-Huan; Fawzi, Nicolas

Citation:

Citation: Wang, Szu-Huan; Zheng, Tongyin; Fawzi, Nicolas. "Structure and interactions of prion-like domains in transcription factor Efg1 phase separation" Biophys. J. 123, 1481-1493 (2024).
PubMed: 38297837

Assembly members:

Assembly members:
entity_1, polymer, 181 residues, Formula weight is not available

Natural source:

Natural source: Common Name: budding yeasts Taxonomy ID: 5476 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Candida albicans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSTYSIPYYNQMNGNYNNGM PQQTTAANQQAFPQQQQPTT TGNASQQQQQAAATAAAAAV QQPYNYMFYQQQGQPGQQTG QTAGQQQQQQQQQQQYDYNT YNRYQYPAATSQGNYYQQTI PNQLSQPQPQHYNGSNRNYT SAPSGAPIPSNSTSGPSQQP PLPGQQAVPIPPHVSTMQQP T

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	510
15N chemical shifts	154
1H chemical shifts	154

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Efg1 PrLD	1

Entities:

Entity 1, Efg1 PrLD 181 residues - Formula weight is not available

1

MET

SER

THR

TYR

SER

ILE

PRO

TYR

ASN

2

GLN

MET

ASN

GLY

ASN

TYR

ASN

GLY

MET

3

PRO

GLN

THR

ALA

ASN

GLN

4

ALA

PHE

PRO

GLN

PRO

THR

5

THR

GLY

ASN

ALA

SER

GLN

6

ALA

THR

ALA

VAL

7

GLN

PRO

TYR

ASN

TYR

MET

PHE

TYR

GLN

8

GLN

GLY

GLN

PRO

GLY

GLN

THR

GLY

9

GLN

THR

ALA

GLY

GLN

10

GLN

TYR

ASP

TYR

ASN

THR

11

TYR

ASN

ARG

TYR

GLN

TYR

PRO

ALA

THR

12

SER

GLN

GLY

ASN

TYR

GLN

THR

ILE

13

PRO

ASN

GLN

LEU

SER

GLN

PRO

GLN

PRO

GLN

14

HIS

TYR

ASN

GLY

SER

ASN

ARG

ASN

TYR

THR

15

SER

ALA

PRO

SER

GLY

ALA

PRO

ILE

PRO

SER

16

ASN

SER

THR

SER

GLY

PRO

SER

GLN

PRO

17

PRO

LEU

PRO

GLY

GLN

ALA

VAL

PRO

ILE

18

PRO

HIS

VAL

SER

THR

MET

GLN

PRO

19

THR

Samples:

sample_1: Efg1 N-PrLD, [U-100% 13C; U-100% 15N], 100 uM; MES 20 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - data analysis

NMRPipe - processing

NMR spectrometers:

Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks