BMRB Entry 52234

Title:
Backbone Assignments of Sis1 J-domain and Gly-rich region
Deposition date:
2023-12-06
Original release date:
2024-04-12
Authors:
Ciesielski, Szymon; Tonelli, Marco; Craig, Elizabeth
Citation:

Citation: Ciesielski, Szymon; Schilke, Brenda; Stolarska, Milena; Tonelli, Marco; Tomiczek, Bartlomiej; Craig, Elizabeth. "Comparative structural and functional analysis of the glycine-rich regions of Class A and B J-domain protein cochaperones of Hsp70"  FEBS Lett. 598, 1465-1477 (2024).
PubMed: 38529663

Assembly members:

Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSmt3-sis1121

Data sets:
Data typeCount
13C chemical shifts218
15N chemical shifts112
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sis1 J-domain and Gly-rich region (1-121 residues)1

Entities:

Entity 1, Sis1 J-domain and Gly-rich region (1-121 residues) 122 residues - Formula weight is not available

1   SERMETVALLYSGLUTHRLYSLEUTYRASP
2   LEULEUGLYVALSERPROSERALAASNGLU
3   GLNGLULEULYSLYSGLYTYRARGLYSALA
4   ALALEULYSTYRHISPROASPLYSPROTHR
5   GLYASPTHRGLULYSPHELYSGLUILESER
6   GLUALAPHEGLUILELEUASNASPPROGLN
7   LYSARGGLUILETYRASPGLNTYRGLYLEU
8   GLUALAALAARGSERGLYGLYPROSERPHE
9   GLYPROGLYGLYPROGLYGLYALAGLYGLY
10   ALAGLYGLYPHEPROGLYGLYALAGLYGLY
11   PHESERGLYGLYHISALAPHESERASNGLU
12   ASPALAPHEASNILEPHESERGLNPHEPHE
13   GLYGLY

Samples:

sample_1: Sis1 1-121, [U-100% 13C; U-100% 15N], 300 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 5 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 300 mM; pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

VNMRj - collection

NMRPipe - processing

SMILE - processing

NMRFAM-SPARKY - data analysis

PINE - data analysis

APES - data analysis

NMR spectrometers:

  • Bruker AVANCE III 900 MHz
  • Varian VXRS 800 MHz
  • Bruker AVANCE III 750 MHz
  • Bruker AVANCE III 600 MHz
  • Varian VXRS 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks