BMRB Entry 52234

Name: Backbone Assignments of Sis1 J-domain and Gly-rich region
Published: 2024-04-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52234

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Assignments of Sis1 J-domain and Gly-rich region

Deposition date:

2023-12-06

Original release date:

2024-04-12

Authors:

Ciesielski, Szymon; Tonelli, Marco; Craig, Elizabeth

Citation:

Citation: Ciesielski, Szymon; Schilke, Brenda; Stolarska, Milena; Tonelli, Marco; Tomiczek, Bartlomiej; Craig, Elizabeth. "Comparative structural and functional analysis of the glycine-rich regions of Class A and B J-domain protein cochaperones of Hsp70" FEBS Lett. 598, 1465-1477 (2024).
PubMed: 38529663

Assembly members:

Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSmt3-sis1121

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMVKETKLYDLLGVSPSANE QELKKGYRKAALKYHPDKPT GDTEKFKEISEAFEILNDPQ KREIYDQYGLEAARSGGPSF GPGGPGGAGGAGGFPGGAGG FSGGHAFSNEDAFNIFSQFF GG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	218
15N chemical shifts	112
1H chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sis1 J-domain and Gly-rich region (1-121 residues)	1

Entities:

Entity 1, Sis1 J-domain and Gly-rich region (1-121 residues) 122 residues - Formula weight is not available

1

SER

MET

VAL

LYS

GLU

THR

LYS

LEU

TYR

ASP

2

LEU

GLY

VAL

SER

PRO

SER

ALA

ASN

GLU

3

GLN

GLU

LEU

LYS

GLY

TYR

ARG

LYS

ALA

4

ALA

LEU

LYS

TYR

HIS

PRO

ASP

LYS

PRO

THR

5

GLY

ASP

THR

GLU

LYS

PHE

LYS

GLU

ILE

SER

6

GLU

ALA

PHE

GLU

ILE

LEU

ASN

ASP

PRO

GLN

7

LYS

ARG

GLU

ILE

TYR

ASP

GLN

TYR

GLY

LEU

8

GLU

ALA

ARG

SER

GLY

PRO

SER

PHE

9

GLY

PRO

GLY

PRO

GLY

ALA

GLY

10

ALA

GLY

PHE

PRO

GLY

ALA

GLY

11

PHE

SER

GLY

HIS

ALA

PHE

SER

ASN

GLU

12

ASP

ALA

PHE

ASN

ILE

PHE

SER

GLN

PHE

13

GLY

Samples:

sample_1: Sis1 1-121, [U-100% 13C; U-100% 15N], 300 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 5 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 300 mM; pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

VNMRj - collection

NMRPipe - processing

SMILE - processing

NMRFAM-SPARKY - data analysis

PINE - data analysis

APES - data analysis

NMR spectrometers:

Bruker AVANCE III 900 MHz
Varian VXRS 800 MHz
Bruker AVANCE III 750 MHz
Bruker AVANCE III 600 MHz
Varian VXRS 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks