BMRB Entry 52227

Name: egg case protein 3
Published: 2024-12-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52227

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

egg case protein 3

Deposition date:

2023-12-05

Original release date:

2024-12-11

Authors:

Yu, Shuixin; Qin, Ruiqi; Lin, Zhi

Citation:

Citation: Yu, Shuixin; Qin, Ruiqi; Yuan, Wensu; Lin, Zhi. "1H, 15N and 13C resonance assignments of eggcase silk protein 3" Biomol. NMR Assign. 18, 227-232 (2024).
PubMed: 39180712

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: western black widow Taxonomy ID: 256737 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Latrodectus hesperus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHHSSGENLYFQGSES MSSSCTYVDGVQRCTQQKSQ GNVAMSSSQAGYGQGQSYSS NYAATGDSGTGQGGYSSMRS GNPPPFPGNGPFAGAATGPA AAAFDPFGFVFGRR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	242
15N chemical shifts	103
1H chemical shifts	520

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ECP3	1

Entities:

Entity 1, ECP3 114 residues - Formula weight is not available

1

MET

HIS

SER

GLY

2

GLU

ASN

LEU

TYR

PHE

GLN

GLY

SER

GLU

SER

3

MET

SER

CYS

THR

TYR

VAL

ASP

GLY

4

VAL

GLN

ARG

CYS

THR

GLN

LYS

SER

GLN

5

GLY

ASN

VAL

ALA

MET

SER

GLN

ALA

6

GLY

TYR

GLY

GLN

GLY

GLN

SER

TYR

SER

7

ASN

TYR

ALA

THR

GLY

ASP

SER

GLY

THR

8

GLY

GLN

GLY

TYR

SER

MET

ARG

SER

9

GLY

ASN

PRO

PHE

PRO

GLY

ASN

GLY

10

PRO

PHE

ALA

GLY

ALA

THR

GLY

PRO

ALA

11

ALA

PHE

ASP

PRO

PHE

GLY

PHE

VAL

12

PHE

GLY

ARG

Samples:

sample_1: ECP3, [U-99% 13C; U-99% 15N], 0.6 mM; sodium acetate, D4, 5 mM

sample_conditions_1: ionic strength: 5 mM; pH: 4.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
4D time-shared 13C, 15N-edited NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1

Software:

NMRView - chemical shift assignment, data analysis, peak picking

NMRPipe - processing

NMRDraw - data analysis

NMR spectrometers:

Bruker Avance 700 MHz