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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52226
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Tants, Jan-Niklas; Oberstrass, Lasse; Weigand, Julia; Schlundt, Andreas. "Structure and RNA-binding of the helically extended Roquin CCCH-type zinc finger" Nucleic Acids Research 52, 9838-9853 (2024).
PubMed: 38953172
Assembly members:
entity_1, polymer, 48 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETTrx1a
Entity Sequences (FASTA):
entity_1: GAMAHSKYKTYMCRDMKQRG
GCPRGASCTFAHSQEELEKF
RKMNKRLV
Data type | Count |
13C chemical shifts | 208 |
15N chemical shifts | 43 |
1H chemical shifts | 324 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Znf | 1 |
2 | Zn | 2 |
Entity 1, Znf 48 residues - Formula weight is not available
residues 407-410 are artificial, natural sequence starts with His 411
1 | GLY | ALA | MET | ALA | HIS | SER | LYS | TYR | LYS | THR | ||||
2 | TYR | MET | CYS | ARG | ASP | MET | LYS | GLN | ARG | GLY | ||||
3 | GLY | CYS | PRO | ARG | GLY | ALA | SER | CYS | THR | PHE | ||||
4 | ALA | HIS | SER | GLN | GLU | GLU | LEU | GLU | LYS | PHE | ||||
5 | ARG | LYS | MET | ASN | LYS | ARG | LEU | VAL |
Entity 2, Zn - Zn - 65.409 Da.
1 | ZN |
sample_1: Roquin zinc finger, [U-99% 15N], 360 uM; zinc 20 uM; sodium chloride 150 mM; TRIS 25 mM; TCEP 2 mM
sample_2: Roquin zinc finger, [U-99% 13C; U-99% 15N], 740 uM; zinc 20 uM; sodium chloride 150 mM; TRIS 25 mM; TCEP 2 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1D 1H | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_2 | isotropic | sample_conditions_1 |
3D CCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_2 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
3D HBHANH | sample_2 | isotropic | sample_conditions_1 |
3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
3D HNCO | sample_2 | isotropic | sample_conditions_1 |
TOPSPIN v3 - collection, processing
CcpNMR v2.1 - chemical shift assignment
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