BMRB Entry 52210

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52210
MolProbity Validation Chart

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Title:
NMR assignments for ZNF711-7
Deposition date:
2023-11-18
Original release date:
2023-11-22
Authors:
Alexandrescu, Andrei; Rua, Antonio
Citation:

Citation: Rua, Antonio; Alexandrescu, Andrei. "Formerly degenerate seventh zinc finger domain from transcription factor ZNF711 rehabilitated by experimental NMR structure"  Protein Sci. 33, e5149-e5149 (2024).
PubMed: 39180464

Assembly members:

Assembly members:
entity_1, polymer, . residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: YKCEHCPQAFGDERELQRHL DLFQGHK

Data sets:
Data typeCount
13C chemical shifts74
15N chemical shifts25
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Z711-71
2ZN2

Entities:

Entity 1, Z711-7 - Formula weight is not available

The N-terminus is acetylated and the C-terminus is amidated

1   TYRLYSCYSGLUHISCYSPROGLNALAPHE
2   GLYASPGLUARGGLULEUGLNARGHISLEU
3   ASPLEUPHEGLNGLYHISLYS

Entity 2, ZN - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Z711-7 2.4 mM; Zn2+ (divalent zinc ion) 4.4 mM

sample_2: Z711-7 2.4 mM; Zn2+ (divalent zinc ion) 4.4 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0 M; pH: 6.2; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
DOSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_2

Software:

CcpNMR - chemical shift assignment of data processed by iNMR (I don't know why iNMR is not on this list!)

X-PLOR NIH - structure calculation

TALOS-N - dihedral angles

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INOVA 600 MHz
  • Bruker AVANCE NEO 600 MHz

Related Database Links:

UNP Q9Y462

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks