BMRB Entry 52207

Name: SDHAF4 assembly factor of Human Complex II
Published: 2023-11-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52207

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SDHAF4 assembly factor of Human Complex II

Deposition date:

2023-11-10

Original release date:

2023-11-17

Authors:

Sharma, Pankaj; Maklashina, Elena; Voehler, Markus; Balinthova, Sona; Dvorakova, Sarka; Kraus, Michal; Hadrava Vanova, Katerina; Nahacka, Zuzana; Zobalova, Renata; Boukalova, Stepana; Cunatova, Kristyna; Mracek, Tomas; Ghayee, Hans; Pacak, Karel; Rohlena, Jakub; Neuzil, Jiri; Cecchini, Gary; Iverson, Tina

Citation:

Citation: Sharma, Pankaj; Maklashina, Elena; Voehler, Markus; Balinthova, Sona; Dvorakova, Sarka; Kraus, Michal; Hadrava Vanova, Katerina; Nahacka, Zuzana; Zobalova, Renata; Boukalova, Stepana; Cunatova, Kristyna; Mracek, Tomas; Ghayee, Hans; Pacak, Karel; Rohlena, Jakub; Neuzil, Jiri; Cecchini, Gary; Iverson, Tina. "Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners" Nat. Commun. 15, 473-473 (2024).
PubMed: 38212624

Assembly members:

Assembly members:
entity_1, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQT-SDHAF4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MRSHHHHHHENLYFQGIDPF TGSSSSQGGKSELVKQSLKK PKLPEGRFDAPEDSHLEKEP LEKFPDDVNPVTKEKGGPRG PEPTRYGDWERKGRCIDF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list

Data type	Count
13C chemical shifts	262
15N chemical shifts	93
1H chemical shifts	77

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SDHAF4	1

Entities:

Entity 1, SDHAF4 98 residues - Formula weight is not available

1

MET

ARG

SER

HIS

GLU

2

ASN

LEU

TYR

PHE

GLN

GLY

ILE

ASP

PRO

PHE

3

THR

GLY

SER

GLN

GLY

LYS

4

SER

GLU

LEU

VAL

LYS

GLN

SER

LEU

LYS

5

PRO

LYS

LEU

PRO

GLU

GLY

ARG

PHE

ASP

ALA

6

PRO

GLU

ASP

SER

HIS

LEU

GLU

LYS

GLU

PRO

7

LEU

GLU

LYS

PHE

PRO

ASP

VAL

ASN

PRO

8

VAL

THR

LYS

GLU

LYS

GLY

PRO

ARG

GLY

9

PRO

GLU

PRO

THR

ARG

TYR

GLY

ASP

TRP

GLU

10

ARG

LYS

GLY

ARG

CYS

ILE

ASP

PHE

Samples:

sample_1: SDHAF4, [U-100% 13C; U-100% 15N], 9.5 mg/mL; potassium phosphate 20 mM; TCEP 10 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 13C-detected hCONCO	sample_1	isotropic	sample_conditions_1
3D 13C-detected hNNCO	sample_1	isotropic	sample_conditions_1
2D 13C-detected CON	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.6.2 - collection

NMRViewJ v9.2.0-b24 - chemical shift assignment

TALOS+ v3.80F1 Rev 2012.080.14.41 - data analysis

NMR spectrometers:

Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks