BMRB Entry 52206

Name: Rat Metabatropic Glutamate Receptor 2 WT C-Terminal Domain
Published: 2024-04-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52206

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Rat Metabatropic Glutamate Receptor 2 WT C-Terminal Domain

Deposition date:

2023-11-10

Original release date:

2024-04-10

Authors:

Mancinelli, Chiara; Eliezer, David

Citation:

Citation: Mancinelli, Chiara; Marx, Dagan; Gonzalez-Hernandez, Alberto; Mancinelli, Lucia; Khelashvilli, George; Levitz, Joshua; Eliezer, David. "Control of G protein-coupled receptor function via membrane-interacting intrinsically disordered C-terminal domains" BioRxiv, PNAS ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 52 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PQKNVVSHRAPTSRFGSAAP RASANLGQGSGSQLVPTVCN GREVVDSTTSSL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	52
15N chemical shifts	47
1H chemical shifts	47

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rmGR2-CTD	1

Entities:

Entity 1, rmGR2-CTD 52 residues - Formula weight is not available

1

PRO

GLN

LYS

ASN

VAL

SER

HIS

ARG

ALA

2

PRO

THR

SER

ARG

PHE

GLY

SER

ALA

PRO

3

ARG

ALA

SER

ALA

ASN

LEU

GLY

GLN

GLY

SER

4

GLY

SER

GLN

LEU

VAL

PRO

THR

VAL

CYS

ASN

5

GLY

ARG

GLU

VAL

ASP

SER

THR

SER

6

SER

LEU

Samples:

sample_1: rmGR2-CTD, [U-100% 13C; U-100% 15N], 200 um; D2O 10%; H2O 90%; HEPES 20 mM; DTT 2 mM; EDTA 1 mM; sodium chloride 250 mM

sample_conditions_1: ionic strength: 250 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

SPARKY - data analysis

NMRbox - data analysis

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks