BMRB Entry 52202

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52202

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Title:
Rat Metabatropic Glutamate Receptor 3 WT C-Terminal Domain
Deposition date:
2023-11-09
Original release date:
2024-04-12
Authors:
Mancinelli, Chiara; Eliezer, David
Citation:

Citation: Mancinelli, Chiara; Marx, Dagan; Gonzalez-Hernandez, Alberto; Huynh, Kevin; Mancinelli, Lucia; Arefin, Anisul; Khelashvilli, George; Levitz, Joshua; Eliezer, David. "Control of G protein-coupled receptor function via membrane-interacting intrinsically disordered C-terminal domains"  Proc. Natl. Acad. Sci. U. S. A. 121, e2407744121-e2407744121 (2024).
PubMed: 38985766

Assembly members:

Assembly members:
entity_1, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PQKNVVTHRLHLNRFSVSGT ATTYSQSSASTYVPTVANGR EVLDSTTSSL

Data sets:
Data typeCount
13C chemical shifts47
15N chemical shifts44
1H chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rmGR3-CTD1

Entities:

Entity 1, rmGR3-CTD 50 residues - Formula weight is not available

1   PROGLNLYSASNVALVALTHRHISARGLEU
2   HISLEUASNARGPHESERVALSERGLYTHR
3   ALATHRTHRTYRSERGLNSERSERALASER
4   THRTYRVALPROTHRVALALAASNGLYARG
5   GLUVALLEUASPSERTHRTHRSERSERLEU

Samples:

sample_1: rmGR3-CTD, [U-100% 13C; U-100% 15N], 200 um; D2O 10%; H2O 90%; HEPES 20 mM; DTT 2 mM; EDTA 1 mM; sodium chloride 250 mM

sample_conditions_1: ionic strength: 250 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

SPARKY - data analysis

NMRbox - data analysis

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks