BMRB Entry 52197

Name: Partial solid-state NMR 13C, 15N resonance assignments of the two conformations of Bj-SemiSWEET in native celular membranes
Published: 2024-01-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52197

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Partial solid-state NMR 13C, 15N resonance assignments of the two conformations of Bj-SemiSWEET in native celular membranes

Deposition date:

2023-10-31

Original release date:

2024-01-04

Authors:

Xie, Huayong; Gan, yuefang; Yang, jun

Citation:

Citation: Zhang, Yan; Gan, Yuefang; Zhao, Weijing; Zhang, Xuning; Zhao, Yongxiang; Xie, Huayong; Yang, Jun. "Membrane Protein Structures in Native Cellular Membranes Revealed by Solid-State NMR Spectroscopy" JACS Au. 3, 3412-3423 (2023).
PubMed: 38155644

Assembly members:

Assembly members:
entity_1, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bradyrhizobium Taxonomy ID: 374 Superkingdom: Bacteria Kingdom: not available Genus/species: Bradyrhizobium not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSD005

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDPFLIKLIGFAAATCTTVA YAPQFIKVLKTRSARDISLG MFLVMVLGLALWLIYGLLSG DAPLIASNAVTMLLAGGILV MKLRYG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	620
15N chemical shifts	170

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Bj-SemiSWEET, conformer A	1
2	Bj-SemiSWEET, conformer B	1

Entities:

Entity 1, Bj-SemiSWEET, conformer A 86 residues - Formula weight is not available

NCBI Reference Sequence: WP_011089200.1

1

MET

ASP

PRO

PHE

LEU

ILE

LYS

LEU

ILE

GLY

2

PHE

ALA

THR

CYS

THR

VAL

ALA

3

TYR

ALA

PRO

GLN

PHE

ILE

LYS

VAL

LEU

LYS

4

THR

ARG

SER

ALA

ARG

ASP

ILE

SER

LEU

GLY

5

MET

PHE

LEU

VAL

MET

VAL

LEU

GLY

LEU

ALA

6

LEU

TRP

LEU

ILE

TYR

GLY

LEU

SER

GLY

7

ASP

ALA

PRO

LEU

ILE

ALA

SER

ASN

ALA

VAL

8

THR

MET

LEU

ALA

GLY

ILE

LEU

VAL

9

MET

LYS

LEU

ARG

TYR

GLY

Samples:

sample_1: Bj-SemiSWEET, [U-13C; U-15N], 0.7 w/w

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
NCACX	sample_1	isotropic	sample_conditions_1
NCOCX	sample_1	isotropic	sample_conditions_1
CONCA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - data analysis

NMR spectrometers:

Bruker AVANCE III 800 MHz