BMRB Entry 52194

Name: INCENP-complex
Published: 2024-05-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52194

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

INCENP-complex

Deposition date:

2023-10-26

Original release date:

2024-05-20

Authors:

Sako, Kouske; Furukawa, Ayako; Nozawa, Ryu-Suke; Kurita, Jun-ichi; Nishimura, Yoshifumi; Hirota, Toru

Citation:

Citation: Sako, Kosuke; Furukawa, Ayako; Nozawa, Ryu-Suke; Kurita, Jun-Ichi; Nishimura, Yoshifumi; Hirota, Toru. "Bipartite binding interface recruiting HP1 to chromosomal passenger complex at inner centromeres" J. Cell Biol. 223, e202312021-e202312021 (2024).
PubMed: 38781028

Assembly members:

Assembly members:
entity_1, polymer, 33 residues, Formula weight is not available
entity_2, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: HTQCQLVPVVEIGISERQNA EQHVTQLMSTEPL
entity_2: NDIARGFERGLEPEKIIGAT DSCGDLMFLMKWKDTDEADL VLAKEANVKCPQIVIAFYEE RLTWHAYPEDAENKEKETAK S

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	24
1H chemical shifts	24

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	INCENP	1
2	HP1_CSD	2

Entities:

Entity 1, INCENP 33 residues - Formula weight is not available

1

HIS

THR

GLN

CYS

GLN

LEU

VAL

PRO

VAL

2

GLU

ILE

GLY

ILE

SER

GLU

ARG

GLN

ASN

ALA

3

GLU

GLN

HIS

VAL

THR

GLN

LEU

MET

SER

THR

4

GLU

PRO

LEU

Entity 2, HP1_CSD 81 residues - Formula weight is not available

1

ASN

ASP

ILE

ALA

ARG

GLY

PHE

GLU

ARG

GLY

2

LEU

GLU

PRO

GLU

LYS

ILE

GLY

ALA

THR

3

ASP

SER

CYS

GLY

ASP

LEU

MET

PHE

LEU

MET

4

LYS

TRP

LYS

ASP

THR

ASP

GLU

ALA

ASP

LEU

5

VAL

LEU

ALA

LYS

GLU

ALA

ASN

VAL

LYS

CYS

6

PRO

GLN

ILE

VAL

ILE

ALA

PHE

TYR

GLU

7

ARG

LEU

THR

TRP

HIS

ALA

TYR

PRO

GLU

ASP

8

ALA

GLU

ASN

LYS

GLU

LYS

GLU

THR

ALA

LYS

9

SER

Samples:

sample_1: INCENP, [U-13C; U-15N], 0.1 mM; HP1_CSD 0.15 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

MAGRO - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks