BMRB Entry 52186
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52186
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Krzysiak, Troy; Choi, You-Jin; Kim, Yong Joon; Yang, Yunhan; DeHaven, Christopher; Thompson, Lariah; Ponticelli, Ryan; Mermigos, Mara; Thomas, Laurel; Marquez, Andrea; Sipula, Ian; Kemper, Jongsook Kim; Jurczak, Michael; Thomas, Gary; Gronenborn, Angela. "Inhibitory protein-protein interactions of the SIRT1 deacetylase are choreographed by post-translational modification" Protein Sci. 33, e4938-e4938 (2024).
PubMed: 38533551
Assembly members:
entity_1, polymer, 72 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41
Entity Sequences (FASTA):
entity_1: SEFGEKQRVFTGIVTSLHDY
FGVVDEEVFFQLSVVKGRLP
QLGEKVLVKAAYNPGQAVPW
NAVKVQTLSNQP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 183 |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 65 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DBC1 52-120 | 1 |
Entities:
Entity 1, DBC1 52-120 72 residues - Formula weight is not available
| 1 | SER | GLU | PHE | GLY | GLU | LYS | GLN | ARG | VAL | PHE | ||||
| 2 | THR | GLY | ILE | VAL | THR | SER | LEU | HIS | ASP | TYR | ||||
| 3 | PHE | GLY | VAL | VAL | ASP | GLU | GLU | VAL | PHE | PHE | ||||
| 4 | GLN | LEU | SER | VAL | VAL | LYS | GLY | ARG | LEU | PRO | ||||
| 5 | GLN | LEU | GLY | GLU | LYS | VAL | LEU | VAL | LYS | ALA | ||||
| 6 | ALA | TYR | ASN | PRO | GLY | GLN | ALA | VAL | PRO | TRP | ||||
| 7 | ASN | ALA | VAL | LYS | VAL | GLN | THR | LEU | SER | ASN | ||||
| 8 | GLN | PRO |
Samples:
sample_1: DBC1 52-120, [U-100% 13C; U-100% 15N], 75 uM; HEPES 20 mM; NaCl 100 mM; TCEP 0.5 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCOCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
CARA - chemical shift assignment
NMR spectrometers:
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks