BMRB Entry 52180

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52180

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Title:
SurA Ile and Met methyl assignments
Deposition date:
2023-10-19
Original release date:
2024-07-31
Authors:
Schiffrin, Bob
Citation:

Citation: Schiffrin, Bob. "SurA Ile and Met methyl assignments"  .

Assembly members:

Assembly members:
entity_1, polymer, 428 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGENLYFQG APQVVDKVAAVVNNGVVLES DVDGLMQSVKLNAAQARQQL PDDATLRHQIMERLIMDQII LQMGQKMGVKISDEQLDQAI ANIAKQNNMTLDQMRSRLAY DGLNYNTYRNQIRKEMIISE VRNNEVRRRITILPQEVESL AQQVGNQNDASTELNLSHIL IPLPENPTSDQVNEAESQAR AIVDQARNGADFGKLAIAHS ADQQALNGGQMGWGRIQELP GIFAQALSTAKKGDIVGPIR SGVGFHILKVNDLRGESKNI SVTEVHARHILLKPSPIMTD EQARVKLEQIAADIKSGKTT FAAAAKEFSQDPGSANQGGD LGWATPDIFDPAFRDALTRL NKGQMSAPVHSSFGWHLIEL LDTRNVDKTDAAQKDRAYRM LMNRKFSEEAASWMQEQRAS AYVKILSN

Data sets:
Data typeCount
13C chemical shifts43
1H chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SurA1

Entities:

Entity 1, SurA 428 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYGLUASNLEUTYRPHEGLNGLY
3   ALAPROGLNVALVALASPLYSVALALAALA
4   VALVALASNASNGLYVALVALLEUGLUSER
5   ASPVALASPGLYLEUMETGLNSERVALLYS
6   LEUASNALAALAGLNALAARGGLNGLNLEU
7   PROASPASPALATHRLEUARGHISGLNILE
8   METGLUARGLEUILEMETASPGLNILEILE
9   LEUGLNMETGLYGLNLYSMETGLYVALLYS
10   ILESERASPGLUGLNLEUASPGLNALAILE
11   ALAASNILEALALYSGLNASNASNMETTHR
12   LEUASPGLNMETARGSERARGLEUALATYR
13   ASPGLYLEUASNTYRASNTHRTYRARGASN
14   GLNILEARGLYSGLUMETILEILESERGLU
15   VALARGASNASNGLUVALARGARGARGILE
16   THRILELEUPROGLNGLUVALGLUSERLEU
17   ALAGLNGLNVALGLYASNGLNASNASPALA
18   SERTHRGLULEUASNLEUSERHISILELEU
19   ILEPROLEUPROGLUASNPROTHRSERASP
20   GLNVALASNGLUALAGLUSERGLNALAARG
21   ALAILEVALASPGLNALAARGASNGLYALA
22   ASPPHEGLYLYSLEUALAILEALAHISSER
23   ALAASPGLNGLNALALEUASNGLYGLYGLN
24   METGLYTRPGLYARGILEGLNGLULEUPRO
25   GLYILEPHEALAGLNALALEUSERTHRALA
26   LYSLYSGLYASPILEVALGLYPROILEARG
27   SERGLYVALGLYPHEHISILELEULYSVAL
28   ASNASPLEUARGGLYGLUSERLYSASNILE
29   SERVALTHRGLUVALHISALAARGHISILE
30   LEULEULYSPROSERPROILEMETTHRASP
31   GLUGLNALAARGVALLYSLEUGLUGLNILE
32   ALAALAASPILELYSSERGLYLYSTHRTHR
33   PHEALAALAALAALALYSGLUPHESERGLN
34   ASPPROGLYSERALAASNGLNGLYGLYASP
35   LEUGLYTRPALATHRPROASPILEPHEASP
36   PROALAPHEARGASPALALEUTHRARGLEU
37   ASNLYSGLYGLNMETSERALAPROVALHIS
38   SERSERPHEGLYTRPHISLEUILEGLULEU
39   LEUASPTHRARGASNVALASPLYSTHRASP
40   ALAALAGLNLYSASPARGALATYRARGMET
41   LEUMETASNARGLYSPHESERGLUGLUALA
42   ALASERTRPMETGLNGLUGLNARGALASER
43   ALATYRVALLYSILELEUSERASN

Samples:

sample_1: SurA, [U-2H], 52.5 uM; D2O 5%; Tris-HCl 20 mM; EDTA 5 mM

sample_conditions_1: ionic strength: 60 mM; pH: 8; pressure: 1 atm; temperature: 25 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.5 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 950 MHz