BMRB Entry 52169

Title:
Clostridioides peptidoglycan fragment dimer
Deposition date:
2023-10-11
Original release date:
2023-10-17
Authors:
Galley, Nicola; Williamson, Mike; Mesnage, Stephane
Citation:

Citation: Galley, Nicola; Greetham, Darren; Alaman-Zarate, Marcel; Williamson, Mike; Evans, Caroline; Spittal, William; Buddle, Jessica; Freeman, Jane; Davis, Georgina; Dickman, Mark; Wilcox, Mark; Lovering, Andrew; Fagan, Robert; Mesnage, Stephane. "Clostridioides difficile canonical L,D-transpeptidases catalyze a novel type of peptidoglycan cross-links and are not required for beta-lactam resistance"  J. Biol. Chem. 300, 105529-105529 (2023).
PubMed: 38043796

Assembly members:

Assembly members:
entity_1, polymer, 5 residues, 1704.7 Da.

Natural source:

Natural source:   Common Name: Clostridioides difficile   Taxonomy ID: 1496   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridioides difficile

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XXXXX

Data sets:
Data typeCount
1H chemical shifts34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GlcNAc-MurNAc-D-Ala-D-isoGlu-diamino pimelic acid dimer1

Entities:

Entity 1, GlcNAc-MurNAc-D-Ala-D-isoGlu-diamino pimelic acid dimer 5 residues - 1704.7 Da.

1   NAGMURDALDGLAPI

Samples:

sample_1: GlcNAc-MurNAc-D-Ala-D-isoGlu-diamino pimelic acid dimer 0.1 ± 0.03 mM

sample_conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.5 - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz