BMRB Entry 52162

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52162

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Title:
Human Rev1-BRCT Domain
Deposition date:
2023-10-05
Original release date:
2023-10-17
Authors:
Arianna, Gianluca; Korzhnev, Dmitry
Citation:

Citation: Arianna, Gianluca; Korzhnev, Dmitry. "Backbone assignment of the human Rev1-BRCT Domain"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
entity_1, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TSSTIFSGVAIYVNGYTDPS AEELRKLMMLHGGQYHVYYS RSKTTHIIATNLPNAKIKEL KGEKVIRPEWIVESIKAGRL LSYIPYQLYT

Data sets:
Data typeCount
13C chemical shifts241
15N chemical shifts78
1H chemical shifts78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rev1-BRCT1

Entities:

Entity 1, Rev1-BRCT 90 residues - Formula weight is not available

1   THRSERSERTHRILEPHESERGLYVALALA
2   ILETYRVALASNGLYTYRTHRASPPROSER
3   ALAGLUGLULEUARGLYSLEUMETMETLEU
4   HISGLYGLYGLNTYRHISVALTYRTYRSER
5   ARGSERLYSTHRTHRHISILEILEALATHR
6   ASNLEUPROASNALALYSILELYSGLULEU
7   LYSGLYGLULYSVALILEARGPROGLUTRP
8   ILEVALGLUSERILELYSALAGLYARGLEU
9   LEUSERTYRILEPROTYRGLNLEUTYRTHR

Samples:

sample_1: EDTA 1 mM; DTT 5 mM; sodium azide 0.05 % v/v; sodium chloride 67.5 mM; potassium chloride 1.3 mM; sodium phosphate 4.9 mM; potassium phosphate 1 mM; Rev1-BRCT, [U-99% 13C; U-99% 15N], 1.3 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 307.75 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
(HBHA)CBCA(CO)NNHsample_1isotropicsample_conditions_1
HBHA(CBCACO)NNHsample_1isotropicsample_conditions_1
HHNsample_1isotropicsample_conditions_1
CCCTOCSY(CO)NNHsample_1isotropicsample_conditions_1
HCCTOCSY(CO)NNHsample_1isotropicsample_conditions_1
3D 15N-separated NOESYsample_1isotropicsample_conditions_1

Software:

NMRbox - processing

NMRPipe - processing

NMRDraw - processing

NMRFAM-SPARKY - chemical shift assignment

SPARTA+ - chemical shift calculation

NMR spectrometers:

  • Bruker Ascend 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks