BMRB Entry 52151

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52151

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Title:
Backbone and sidechain chemical shift assignment of DRB3 dsRBD2
Deposition date:
2023-09-26
Original release date:
2024-09-26
Authors:
Paul, Jaydeep; Deshmukh, Mandar V.
Citation:

Citation: Paul, Jaydeep; Deshmukh, Mandar V.. "Chemical shift assignment of dsRBD1 and dsRBD2 of Arabidopsis thaliana DRB3, an essential protein involved in RNAi-mediated antiviral defense"  Biomol. NMR Assign. 18, 99-9104 (2024).
PubMed: 38668800

Assembly members:

Assembly members:
entity_1, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDETGIYKNLLQETAHRAGL DLPVYTSVRSGPGHIPTFSC TVELAGMSFNGESAKTKKQA EKNAAIAAWFSLRKMPRLDP LRGEEKEQEIVARVLSRFRP KEVKLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts87
1H chemical shifts576

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DRB3 dsRBD2 (83-185)1

Entities:

Entity 1, DRB3 dsRBD2 (83-185) 112 residues - Formula weight is not available

1   METASPGLUTHRGLYILETYRLYSASNLEU
2   LEUGLNGLUTHRALAHISARGALAGLYLEU
3   ASPLEUPROVALTYRTHRSERVALARGSER
4   GLYPROGLYHISILEPROTHRPHESERCYS
5   THRVALGLULEUALAGLYMETSERPHEASN
6   GLYGLUSERALALYSTHRLYSLYSGLNALA
7   GLULYSASNALAALAILEALAALATRPPHE
8   SERLEUARGLYSMETPROARGLEUASPPRO
9   LEUARGGLYGLUGLULYSGLUGLNGLUILE
10   VALALAARGVALLEUSERARGPHEARGPRO
11   LYSGLUVALLYSLEUGLUHISHISHISHIS
12   HISHIS

Samples:

sample_1: DRB3 dsRBD2 (83-185) 450 uM; Pottasium phosphate buffer 50 mM; NaCl 50 mM; Na2SO4 50 mM

sample_2: DRB3 dsRBD2 (83-185), [U-98% 15N], 450 uM; Pottasium phosphate buffer 50 mM; NaCl 50 mM; Na2SO4 50 mM

sample_3: DRB3 dsRBD2 (83-185), [U-98% 13C; U-98% 15N], 450 uM; Pottasium phosphate buffer 50 mM; NaCl 50 mM; Na2SO4 50 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HN(CA)COsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HN(COCA)CBsample_3isotropicsample_conditions_1
3D HN(CA)CBsample_3isotropicsample_conditions_1
3D H(CCO)NHsample_3isotropicsample_conditions_1
3D (H)C(CO)NH-TOCSYsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
3D HNHAsample_3isotropicsample_conditions_1
3D 1H(15N)-1H(13C) NOESYsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HN(CA)CBsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HN(COCA)CBsample_3isotropicsample_conditions_1

Software:

TOPSPIN v4.1 - collection, data analysis, processing

CARA v1.9.1 - chemical shift assignment, chemical shift calculation, data analysis, peak picking

TALOS-N - chemical shift calculation

CSI v3.0 - chemical shift calculation

MddNMR - data analysis

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Related Database Links:

UNP Q9LJF5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks