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BMRB Entry 52144 BMRB - Biological Magnetic Resonance Bank

BMRB Entry 52144

BMRB Entry DOI: doi:10.13018/BMR52144

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All files associated with the entry

Title:
Backbone 1H, 13C and 15N Chemical Shift Assignment of Mouse Siderocalin (Lipocalin 2)
Deposition date:
2023-09-20
Original release date:
2024-06-24
Authors:
Moeller, Johanna; Bozhanova, Nina; Voehler, Markus; Meiler, Jens; Schoeder, Clara
Citation:

Citation: Moeller, Johanna; Bozhanova, Nina; Voehler, Markus; Meiler, Jens; Schoeder, Clara. "Backbone chemical shift and secondary structure assignments for mouse siderocalin"  Biomol. NMR Assign. 18, 79-84 (2024).
PubMed: 38564159

Assembly members:

Assembly members:
entity_1, polymer, 180 residues, 20874 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QDSTQNLIPAPSLLTVPLQP DFRSDQFRGRWYVVGLAGNA VQKKTEGSFTMYSTIYELQE NNSYNVTSILVRDQDQGCRY WIRTFVPSSRAGQFTLGNMH RYPQVQSYNVQVATTDYNQF AMVFFRKTSENKQYFKITLY GRTKELSPELKERFTRFAKS LGLKDDNIIFSVPTDQCIDN

Data sets:
Data typeCount
13C chemical shifts280
15N chemical shifts150
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1siderocalin1

Entities:

Entity 1, siderocalin 180 residues - 20874 Da.

1   GLNASPSERTHRGLNASNLEUILEPROALA
2   PROSERLEULEUTHRVALPROLEUGLNPRO
3   ASPPHEARGSERASPGLNPHEARGGLYARG
4   TRPTYRVALVALGLYLEUALAGLYASNALA
5   VALGLNLYSLYSTHRGLUGLYSERPHETHR
6   METTYRSERTHRILETYRGLULEUGLNGLU
7   ASNASNSERTYRASNVALTHRSERILELEU
8   VALARGASPGLNASPGLNGLYCYSARGTYR
9   TRPILEARGTHRPHEVALPROSERSERARG
10   ALAGLYGLNPHETHRLEUGLYASNMETHIS
11   ARGTYRPROGLNVALGLNSERTYRASNVAL
12   GLNVALALATHRTHRASPTYRASNGLNPHE
13   ALAMETVALPHEPHEARGLYSTHRSERGLU
14   ASNLYSGLNTYRPHELYSILETHRLEUTYR
15   GLYARGTHRLYSGLULEUSERPROGLULEU
16   LYSGLUARGPHETHRARGPHEALALYSSER
17   LEUGLYLEULYSASPASPASNILEILEPHE
18   SERVALPROTHRASPGLNCYSILEASPASN

Samples:

sample_1: Mouse siderocalin, [U-100% 13C; U-100% 15N], 500 uM; D2O, [U-100% 2H], 5%; DSS 1 mM; sodium phosphate 100 mM

sample_conditions_1: ionic strength: 0.6 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

CcpNMR v3.1.1 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

NCBI NP_032517.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks