BMRB Entry 52141
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52141
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Sahoo, Bikash; Subramanian, Vivekanandan; Bardwell, James. "Backbone 1H, 13C, and 15N chemical shift assignments for human SERF2" Biomol. NMR Assign. ., .-. (2024).
PubMed: 38466543
Assembly members:
entity_1, polymer, 59 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
entity_1: MTRGNQRELARQKNMKKQSD
SVKGKRRDDGLSAAARKQRD
SEIMQQKQKKANEKKEEPK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 150 |
| 15N chemical shifts | 50 |
| 1H chemical shifts | 278 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human SERF2 | 1 |
Entities:
Entity 1, human SERF2 59 residues - Formula weight is not available
| 1 | MET | THR | ARG | GLY | ASN | GLN | ARG | GLU | LEU | ALA | ||||
| 2 | ARG | GLN | LYS | ASN | MET | LYS | LYS | GLN | SER | ASP | ||||
| 3 | SER | VAL | LYS | GLY | LYS | ARG | ARG | ASP | ASP | GLY | ||||
| 4 | LEU | SER | ALA | ALA | ALA | ARG | LYS | GLN | ARG | ASP | ||||
| 5 | SER | GLU | ILE | MET | GLN | GLN | LYS | GLN | LYS | LYS | ||||
| 6 | ALA | ASN | GLU | LYS | LYS | GLU | GLU | PRO | LYS |
Samples:
sample_1: Human SERF2, [U-100% 13C; U-100% 15N], 1.2 mM; d3-Sodium acetate 20 mM; KCl 20 mM; EDTA 2 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 5.5; pressure: 1 atm; temperature: 277.1 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY v1.470 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks