BMRB Entry 52138

Name: Backbone assignment of the R178Q mutant of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1)
Published: 2024-01-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52138

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of the R178Q mutant of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1)

Deposition date:

2023-09-19

Original release date:

2024-01-09

Authors:

Chiang, Tsung-Sheng; Lai, Chih-Hsuan; Hsu, Shang-Te Danny

Citation:

Citation: Kenny, Sebastian; Lai, Chih-Hsuan; Chiang, Tsung-Sheng; Brown, Kwame; Hewitt, Chad; Krabill, Aaron; Chang, Hao-Ting; Wang, Yong-Sheng; Flaherty, Daniel; Danny Hsu, Shang-Te; Das, Chittaranjan. "Altered protein dynamics and a more reactive catalytic cysteine in a neurodegeneration-associated UCHL1 mutant" J. Mol. Biol. 436, 168438-168438 (2024).
PubMed: 38185323

Assembly members:

Assembly members:
entity_1, polymer, 231 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQLKPMEINPEMLNKVLSRL GVAGQWRFVDVLGLEEESLG SVPAPACALLLLFPLTAQHE NFRKKQIEELKGQEVSPKVY FMKQTIGNSCGTIGLIHAVA NNQDKLGFEDGSVLKQFLSE TEKMSPEDRAKCFEKNEAIQ AAHDAVAQEGQCRVDDKVNF HFILFNNVDGHLYELDGQMP FPVNHGASSEDTLLKDAAKV CREFTEREQGEVRFSAVALC KAALEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	506
15N chemical shifts	172
1H chemical shifts	172

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UCHL1_R178Q	1

Entities:

Entity 1, UCHL1_R178Q 231 residues - Formula weight is not available

1

MET

GLN

LEU

LYS

PRO

MET

GLU

ILE

ASN

PRO

2

GLU

MET

LEU

ASN

LYS

VAL

LEU

SER

ARG

LEU

3

GLY

VAL

ALA

GLY

GLN

TRP

ARG

PHE

VAL

ASP

4

VAL

LEU

GLY

LEU

GLU

SER

LEU

GLY

5

SER

VAL

PRO

ALA

PRO

ALA

CYS

ALA

LEU

6

LEU

PHE

PRO

LEU

THR

ALA

GLN

HIS

GLU

7

ASN

PHE

ARG

LYS

GLN

ILE

GLU

LEU

8

LYS

GLY

GLN

GLU

VAL

SER

PRO

LYS

VAL

TYR

9

PHE

MET

LYS

GLN

THR

ILE

GLY

ASN

SER

CYS

10

GLY

THR

ILE

GLY

LEU

ILE

HIS

ALA

VAL

ALA

11

ASN

GLN

ASP

LYS

LEU

GLY

PHE

GLU

ASP

12

GLY

SER

VAL

LEU

LYS

GLN

PHE

LEU

SER

GLU

13

THR

GLU

LYS

MET

SER

PRO

GLU

ASP

ARG

ALA

14

LYS

CYS

PHE

GLU

LYS

ASN

GLU

ALA

ILE

GLN

15

ALA

HIS

ASP

ALA

VAL

ALA

GLN

GLU

GLY

16

GLN

CYS

ARG

VAL

ASP

LYS

VAL

ASN

PHE

17

HIS

PHE

ILE

LEU

PHE

ASN

VAL

ASP

GLY

18

HIS

LEU

TYR

GLU

LEU

ASP

GLY

GLN

MET

PRO

19

PHE

PRO

VAL

ASN

HIS

GLY

ALA

SER

GLU

20

ASP

THR

LEU

LYS

ASP

ALA

LYS

VAL

21

CYS

ARG

GLU

PHE

THR

GLU

ARG

GLU

GLN

GLY

22

GLU

VAL

ARG

PHE

SER

ALA

VAL

ALA

LEU

CYS

23

LYS

ALA

LEU

GLU

HIS

24

HIS

Samples:

sample_1: R178Q mutant of ubiquitin carboxy terminal hydrolase L1 (UCH-L1), [U-13C; U-15N], 1 mM; EDTA 0.5 mM; TCEP 1 mM; NaN3 0.02%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks