BMRB Entry 52130

Name: Backbone assignment of C-teminus of yeast Oxa1
Published: 2023-11-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52130

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of C-teminus of yeast Oxa1

Deposition date:

2023-09-17

Original release date:

2023-11-15

Authors:

Liu, Yong; Yang, Jing; Ruan, Maosen; Zhang, Huiqin; Wang, Junfeng; Li, Yunyan

Citation:

Citation: Liu, Yong; Yang, Jing; Ruan, Maosen; Zhang, Huiqin; Wang, Junfeng; Li, Yunyan. "NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes" Int. J. Mol. Sci . 24, 14657-14657 (2023).
PubMed: 37834108

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET14b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MWVRSKLKITEVAKPRTPIA GASPTENMGIFQSLKHNIQK ARDQAERRQLMQDNEKKLQE SFKEKRQNSKIKIVHKSNFI NNKK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	521
15N chemical shifts	151
1H chemical shifts	872

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Oxa1-CTD	1

Entities:

Entity 1, Oxa1-CTD 104 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

TRP

VAL

ARG

SER

LYS

LEU

LYS

ILE

THR

4

GLU

VAL

ALA

LYS

PRO

ARG

THR

PRO

ILE

ALA

5

GLY

ALA

SER

PRO

THR

GLU

ASN

MET

GLY

ILE

6

PHE

GLN

SER

LEU

LYS

HIS

ASN

ILE

GLN

LYS

7

ALA

ARG

ASP

GLN

ALA

GLU

ARG

GLN

LEU

8

MET

GLN

ASP

ASN

GLU

LYS

LEU

GLN

GLU

9

SER

PHE

LYS

GLU

LYS

ARG

GLN

ASN

SER

LYS

10

ILE

LYS

ILE

VAL

HIS

LYS

SER

ASN

PHE

ILE

11

ASN

LYS

Samples:

sample_1: Oxa1-CTD, [U-95% 15N], 0.045 mM; Oxa1-CTD, [U-13C; U-15N], 0.5 mM; HEPES 20 mM; NaCl 75 mM; MgCl2 20 mM; b-mercaptoethanol 5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks