BMRB Entry 5213

Name: Copper Trafficking: The Solution Structure of Bacillus subtilis CopZ
Published: 2002-02-14

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PDB ID: 1k0v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5213
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Copper Trafficking: The Solution Structure of Bacillus subtilis CopZ

Deposition date:

2001-11-20

Original release date:

2002-02-14

Authors:

Banci, L.; Bertini, I.; Del Conte, R.; Markey, J.; Ruiz-Duenas, J.

Citation:

Citation: Banci, L.; Bertini, I.; Del Conte, R.; Markey, J.; Ruiz-Duenas, F.. "Copper Trafficking: The Solution Structure of Bacillus subtilis CopZ" Biochemistry 40, 15660-15668 (2001).
PubMed: 11747441

Assembly members:

Assembly members:
CopZ from Bacillus subtilis, polymer, 73 residues, 7826 Da.
COPPER (I) ION, non-polymer, 63.546 Da.

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Bacillus subtilis Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CopZ from Bacillus subtilis: MEQKTLQVEGMSCQHCVKAV ETSVGELDGVSAVHVNLEAG KVDVSFDADKVSVKDIADAI EDQGYDVAKIEGR

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
15N chemical shifts	73
1H chemical shifts	461

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CopZ from Bacillus subtilis	1
2	COPPER (I) ION	2

Entities:

Entity 1, CopZ from Bacillus subtilis 73 residues - 7826 Da.

1

MET

GLU

GLN

LYS

THR

LEU

GLN

VAL

GLU

GLY

2

MET

SER

CYS

GLN

HIS

CYS

VAL

LYS

ALA

VAL

3

GLU

THR

SER

VAL

GLY

GLU

LEU

ASP

GLY

VAL

4

SER

ALA

VAL

HIS

VAL

ASN

LEU

GLU

ALA

GLY

5

LYS

VAL

ASP

VAL

SER

PHE

ASP

ALA

ASP

LYS

6

VAL

SER

VAL

LYS

ASP

ILE

ALA

ASP

ALA

ILE

7

GLU

ASP

GLN

GLY

TYR

ASP

VAL

ALA

LYS

ILE

8

GLU

GLY

ARG

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1

CU1

Samples:

sample_1: CopZ from Bacillus subtilis, [U-99% 15N], 2 mM; COPPER (I) ION 2 mM; phosphate buffer 100 mM; DTT trace mM; D2O 10%; H2O 90%

sample_2: CopZ from Bacillus subtilis 2 mM; COPPER (I) ION 2 mM; phosphate buffer 100 mM; DTT trace mM; D2O 10%; H2O 90%

sample_cond_1: pH: 7.0 na; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D TOSCY-HMQC	not available	not available	sample_cond_1
3D NOESY-HSQC	not available	not available	sample_cond_1
3D HNHA	not available	not available	sample_cond_1
2D TOSCY	not available	not available	sample_cond_1
2D NOESY	not available	not available	sample_cond_1
2D HSQC	not available	not available	sample_cond_1

Software:

DYANA v1.5 - solution structure calculation

xwinnmr v2.6 - NMR experiments collection

XEASY v1.3.13 - data analysis

AMBER v5.0 - solution structure refinement

Molmol v2.4 - data analysis

CORMA - data analysis

NMR spectrometers:

Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks