BMRB Entry 52122

Title:
d(5mCpG)3
Deposition date:
2023-09-11
Original release date:
2023-12-27
Authors:
Nichols, Parker; Krall, Jeffrey; Henen, Morkos; Welty, Robb; MacFadden, Andrea; Vicens, Quentin; Vogeli, Beat
Citation:

Citation: Nichols, Parker; Krall, Jeffrey; Henen, Morkos; Welty, Robb; MacFadden, Andrea; Vicens, Quentin; Vogeli, Beat. "Z-Form Adoption of Nucleic Acid is a Multi-Step Process Which Proceeds through a Melted Intermediate"  J. Am. Chem. Soc. 146, 677-694 (2024).
PubMed: 38131335

Assembly members:

Assembly members:
entity_1, polymer, 6 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XGXGXG

Data sets:
Data typeCount
13C chemical shifts38
1H chemical shifts54

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1d(5mCpG)31

Entities:

Entity 1, d(5mCpG)3 6 residues - Formula weight is not available

1   MCYDGMCYDGMCYDG

Samples:

sample_1: d(5mCpG)3 3.1 mM; potassium phosphate 20 mM; NaCl 25 mM; EDTA 0.5 mM

sample_conditions_1: ionic strength: 0.243 M; pH: 6.4; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMRPipe - processing

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz