BMRB Entry 52114

Title:
thermostable WW domain basic scaffold to design functional b-sheet miniproteins
Deposition date:
2023-09-07
Original release date:
2024-06-27
Authors:
Kovermann, Michael
Citation:

Citation: Lindner, Christina; Friemel, Anke; Schwegler, Niklas; Timmermann, Lisa; Pham, Truc Lam; Reusche, Vanessa; Kovermann, Michael; Thomas, Franziska. "Thermostable WW-Domain Scaffold to Design Functional beta-Sheet Miniproteins"  J. Am. Chem. Soc. ., .-. (2024).
PubMed: 38853610

Assembly members:

Assembly members:
entity_1, polymer, 34 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PLPPGWEIRIESGSGRIYYY NSITKTTTWERPRL

Data sets:
Data typeCount
13C chemical shifts123
15N chemical shifts32
1H chemical shifts248

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1b-sheet miniproteins1

Entities:

Entity 1, b-sheet miniproteins 34 residues - Formula weight is not available

1   PROLEUPROPROGLYTRPGLUILEARGILE
2   GLUSERGLYSERGLYARGILETYRTYRTYR
3   ASNSERILETHRLYSTHRTHRTHRTRPGLU
4   ARGPROARGLEU

Samples:

sample_1: cl033 1 mM; NaCl 137 mM; KCl 2.7 mM; Na2HPO4 10 mM; KH2PO4 1.8 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC-TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - chemical shift assignment, collection, data analysis, peak picking

ARIA - structure solution

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks