BMRB Entry 52109

Name: Chemical shifts for IAA17/AXR3 N-terminal
Published: 2023-09-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52109

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Chemical shifts for IAA17/AXR3 N-terminal

Deposition date:

2023-09-01

Original release date:

2023-09-29

Authors:

Ramans-Harborough, Sigurd; Kalverda, Arnout; Manfield, Iain; Thompson, Gary; Kieffer, Martin; Uzunova, Veselina; Quareshy, Mussa; Prusinska, Justyna; Roychoudhry, Suruchi; Hayashi, Ken-ichiro; Napier, Richard; de Genio, Charo; Kepinski, Stephan

Citation:

Citation: Ramans-Harborough, Sigurd; Kalverda, Arnout; Manfield, Iain; Thompson, Gary; Kieffer, Martin; Uzunova, Veselina; Quareshy, Mussa; Prusinska, Justyna; Roychoudhry, Suruchi; Hayashi, Ken-ichiro; Napier, Richard; del Genio, Charo; Kepinski, Stefan. "Intrinsic disorder and conformational coexistence in auxin coreceptors" Proc. Natl. Acad. Sci. U. S. A. 120, e2221286120-e2221286120 (2023).
PubMed: 37756337

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, 14370.27 Da.

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a Novagen

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH NQTSLYKKAGCMMGSVELNL RETELCLGLPGGDTVAPVTG NKRGFSETVDLKLNLNNEPA NKEGSTTHDVVTFDSKEKSA CPKDPAKPPAKAQVVGWPPV RSYRKNVMVSCQK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	615
15N chemical shifts	225
1H chemical shifts	213

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IAA17/AXR3 N-terminal half, chain A	1
2	IAA17/AXR3 N-terminal half, chain B	1

Entities:

Entity 1, IAA17/AXR3 N-terminal half, chain A 133 residues - 14370.27 Da.

The sequence in the deposition starts at -30 Two sets of chemical shifts are reported for around Residue 87 (PRO) chain A 87 trans chain B 87 cis

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

ASN

GLN

THR

SER

LEU

TYR

LYS

ALA

GLY

4

CYS

MET

GLY

SER

VAL

GLU

LEU

ASN

LEU

5

ARG

GLU

THR

GLU

LEU

CYS

LEU

GLY

LEU

PRO

6

GLY

ASP

THR

VAL

ALA

PRO

VAL

THR

GLY

7

ASN

LYS

ARG

GLY

PHE

SER

GLU

THR

VAL

ASP

8

LEU

LYS

LEU

ASN

LEU

ASN

GLU

PRO

ALA

9

ASN

LYS

GLU

GLY

SER

THR

HIS

ASP

VAL

10

VAL

THR

PHE

ASP

SER

LYS

GLU

LYS

SER

ALA

11

CYS

PRO

LYS

ASP

PRO

ALA

LYS

PRO

ALA

12

LYS

ALA

GLN

VAL

GLY

TRP

PRO

VAL

13

ARG

SER

TYR

ARG

LYS

ASN

VAL

MET

VAL

SER

14

CYS

GLN

LYS

Samples:

sample_1: IAA17/AXR3 N-terminal half, [U-99% 13C; U-99% 15N], 290 uM; D2O 5%; NaxPO3 20 mM; NaCl 150 mM; EDTA 3 mM; DTT 10 mM; protease inhibitor cocktail 2 % v/v

sample_conditions_1: ionic strength: 210 mM; pH: 6.0; pressure: 1 atm; temperature: 289.6 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNcoCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D CON	sample_1	isotropic	sample_conditions_1
2D CACO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D CAnCO	sample_1	isotropic	sample_conditions_1

Software:

CCPN Assign v2.4 - chemical shift assignment

NMRPipe vunknown - chemical shift assignment

NMR spectrometers:

Agilent DDX3 600 MHz
Bruker AVIII HD 950 MHz