BMRB Entry 52105

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52105

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Title:
1H and 15N chemical shift assignments of K6-linked di-ubiquitin with chain-terminating mutations
Deposition date:
2023-08-28
Original release date:
2023-08-30
Authors:
Kurbah, Iladeiti; Fushman, David
Citation:

Citation: Kurbah, Iladeiti; Fushman, David. "1H and 15N chemical shift assignments of K6-linked di-ubiquitin with chain-terminating mutations"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 77 residues, 8707.95 Da.
entity_2, polymer, 76 residues, 8620.87 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGRQLEDGRTLSDYN IQKESTLHLVLRLRGGD
entity_2: MQIFVRTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGRQLEDGRTLSDYN IQKESTLHLVLRLRGG

Data sets:
Data typeCount
15N chemical shifts142
1H chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin, Chain A1
2ubiquitin, Chain B2

Entities:

Entity 1, ubiquitin, Chain A 77 residues - 8707.95 Da.

Proximal domain of K6-linked di-ubiquitin

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYARGGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLYASP

Entity 2, ubiquitin, Chain B 76 residues - 8620.87 Da.

Distal domain of K6-linked di-ubiquitin

1   METGLNILEPHEVALARGTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYARGGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Related Database Links:

UNP P0CG47 P0CG47

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