BMRB Entry 52099

Name: backbone 13C and 15N chemical shift assignment of the conductance domain of Influenza A M2 protein in DPhPC proteoliposomes
Published: 2023-11-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52099

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

backbone 13C and 15N chemical shift assignment of the conductance domain of Influenza A M2 protein in DPhPC proteoliposomes

Deposition date:

2023-08-24

Original release date:

2023-11-15

Authors:

Mohr, Swantje; Lange, Adam

Citation:

Citation: Paschke, Ronja; Mohr, Swantje; Lange, Sascha; Lange, Adam; Kozuch, Jacek. "In Situ Spectroscopic Detection of Large-Scale Reorientations of Transmembrane Helices During Influenza A M2 Channel Opening" Angew. Chem., Int. Ed. 62, e202309069-e202309069 (2023).
PubMed: 37733579

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, 5000 Da.

Natural source:

Natural source: Common Name: Haemophilus influenzae Taxonomy ID: 727 Superkingdom: Bacteria Kingdom: not available Genus/species: Haemophilus influenzae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RSNDSSDPLVVAASIIGILH LILWILDRLFFKSIYRFFEH GLK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	98
15N chemical shifts	34

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	M2 monomer A	1
2	M2 monomer B	1

Entities:

Entity 1, M2 monomer A 43 residues - 5000 Da.

1

ARG

SER

ASN

ASP

SER

ASP

PRO

LEU

VAL

2

VAL

ALA

SER

ILE

GLY

ILE

LEU

HIS

3

LEU

ILE

LEU

TRP

ILE

LEU

ASP

ARG

LEU

PHE

4

PHE

LYS

SER

ILE

TYR

ARG

PHE

GLU

HIS

5

GLY

LEU

LYS

Samples:

sample_1: M2 conductance domain, [U-100% 13C; U-100% 15N], 50 % w/w; sodium phosphate 40 mM; glutamate 30 mM; sodium azide 3 mM; 1,2-diphytanoyl-sn-glycero-3-phosphocholine 50 % w/w

sample_conditions_1: pH: 7.8; pressure: 1 atm; temperature: 387 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D (H)NCACB	sample_1	isotropic	sample_conditions_1
3D (H)NCACO	sample_1	isotropic	sample_conditions_1
3D (H)NCOCA	sample_1	isotropic	sample_conditions_1
3D (H)CANCO	sample_1	isotropic	sample_conditions_1
2D (H)NCACX	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1.1 - data collection, initial spectra representation

CcpNMR v3.1.0 - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AVANCE NEO 700 MHz