BMRB Entry 52090

Name: Chemical shifts of Ost4 protein assigned by solid state NMR
Published: 2024-03-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52090

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts of Ost4 protein assigned by solid state NMR

Deposition date:

2023-08-21

Original release date:

2024-03-11

Authors:

Chaudhary, Bharat; Struppe, Jochem; Moktan, Hem; Zoetewey, David; Zhou, Donghua; Mohanty, Smita

Citation:

Citation: Chaudhary, Bharat; Struppe, Jochem; Moktan, Hem; Zoetewey, David; Zhou, Donghua; Mohanty, Smita. "Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR" J. Biomol. NMR 78, 109-117 (2024).
PubMed: 38421550

Assembly members:

Assembly members:
entity_1, polymer, 39 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: GEV2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MISDEQLNSLAITFGIVMMT LIVIYHAVDSTMSPKNRLE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	148
15N chemical shifts	36

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ost4	1

Entities:

Entity 1, Ost4 39 residues - Formula weight is not available

1

MET

ILE

SER

ASP

GLU

GLN

LEU

ASN

SER

LEU

2

ALA

ILE

THR

PHE

GLY

ILE

VAL

MET

THR

3

LEU

ILE

VAL

ILE

TYR

HIS

ALA

VAL

ASP

SER

4

THR

MET

SER

PRO

LYS

ASN

ARG

LEU

GLU

Samples:

sample_1: Ost4, [U-100% 13C; U-100% 15N], 15 mg

sample_conditions_1: pH: 6.5; temperature: 283 K

sample_conditions_2: pH: 6.5; temperature: 230 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C DARR	sample_1	isotropic	sample_conditions_1
3D NCACX	sample_1	isotropic	sample_conditions_1
3D NCOCX	sample_1	isotropic	sample_conditions_1
3D CANcoCX	sample_1	isotropic	sample_conditions_1
2D CHHC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.1 - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AVANCE 600 MHz
Bruker AVANCE 700 MHz